Day 7: Investigating axonal biology using cerebral cortical neurons in a microfluidic culture device.

We have reached day 7 of our investigation into the suitability of Axol hyCCNs for studying axonal biology using microfluidic culture devices. Unfortunately, the major highlights at these point are changing media and checking cells under the microscope while we wait for the assay to come to fruition (a familiar scenario to those experienced in cell culture). Prior to today, we prepared the microfluidic culture device and began the culture of human cerebral cortical neuron with the expectation that the axons would pass through the microgroove within 2 weeks of plating the cells. 

In the last post (Day 5) we saw an axon peeking through the other side and were cautiously optimistic about the progression our neurons had made. Here is what happened on Day 7!

To do:

• Replace the medium with Complete Axol Neural Maintenance Medium.

• Check how the cortical neurons are growing on the microfluidic device. 

RESULT!

We will check back on them in a couple of days to see what happens!!

Find out what happened on previous days:

Day 5: Investigating axonal biology using cerebral cortical neurons in a microfluidic culture device.
Day 3: Investigating axonal biology using cerebral cortical neurons in a microfluidic culture device.
Day 2: Investigating axonal biology using cerebral cortical neurons in a microfluidic culture device.
Day 1: Investigating axonal biology using cerebral cortical neurons in a microfluidic culture device.

Thank you to xona microfluidics for providing us with the culture device for testing.