3D cell culture techniques for culturing neural stem cells and neurons provide highly useful tools for studying neurogenesis, neuropathology, toxicology and effects of new drug targets. They also allow the potential for side by side comparisons of disease state cells vs healthy controls for the study of neurological disorders and diseases. The use of such systems overcomes some of the limitations of standard 2D culture systems in which neural cells are cultured as a monolayer. The recapitulation of the complex microenvironment in which neural cells exists allows 3D culture systems to bridge the gap between traditional cell culture approaches and in vivo models such as transgenic mice. By mimicking the "stem cell niche" and physiological environments both mechanically and spatially, the crosstalk between the cells and environment leads to a closer scenario to what can be seen in the brain. There are number of different approaches to 3D culture of neuronal cells including generation of organoids/spheroids or scaffolding systems such as collagen hydrogels and nanofiber scaffolds.
To investigate the suitability of Axol neural cells for use in a 3D culture system we used TAP Biosystems RAFT technology, a collagen based hydrogel system. The resulting gels were stained using immunocytochemistry and analysed using confocal microscopy.