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Cerebral Cortical Neurons

Overview

With this system, Neural Stem Cell (NSC) populations can be differentiated 
into pure populations of cerebral cortical neurons according to your specifications. All reagents are fully-defined, animal component-free and have been developed together to ensure optimal consistency for your research. Built to be modern, our media formulations incorporate a number of tools validated within the neuroscience field to improve key research areas including electrophysiology, neurite outgrowth/branching and long-term culturing. 

Low-density cultures

High-purity neurons 

Long-term culturing capacity (>50 days)

Maximal adherence.  No cell body clumping

 

Scroll down to discover application workflows, protocols & images

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Choose your Desired Format:

Plastic Multiwells

Microplates

Glass Surface

 
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Workflows

Synchronous Differentiation

1 - No prior expansion of NSCs. Pure population of neurons obtained in under 5 days from thawing. 
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Reagents ​Xeno-free ​Description Protocol
SureBond-XF (ax0041XF)
Neural Differentiation-XF Medium (ax0034-125)
Neural Maintenance-XF Medium (ax0032-500)
- Plating-XF Medium

YES
Achieve >90% pure cerebral cortical neurons in less then 5 days with minimal cell body clumping ​Protocol 1: Synchronous differentiation of Neural Stem Cells (No expansion)

18 hours after thawing Axol Neural Stem Cells in Axol Neural Differentiation-XF Medium ( ax0034-125 ) on Sure Bond-XF coating . Image inverted to highlight neurite processes.  

36 hours after thawing Axol Neural Stem Cells in Axol Neural Differentiation-XF Medium ( ax0034-125 ) on Sure Bond-XF coating . Image inverted to highlight neurite processes.  

54 hours after thawing Axol Neural Stem Cells in Axol Neural Differentiation-XF Medium ( ax0034-125 ) on Sure Bond-XF coating . Image inverted to highlight neurite processes.  

CLICK HERE for high resolution image                           CLICK HERE for high resolution image                           CLICK HERE for high resolution image

Synchronous Differentiation

2 - Brief expansion of NSCs prior to inducing differentiation. 
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Reagents Xeno-free Description Protocol
Surebond-XF (ax0041XF)
Neural Expansion XF Medium (ax0030-500)
Neural Differentiation-XF Medium (ax0034-125)
Neural Maintenance-XF Medium (ax0032-500)
- Plating-XF Medium
- Unlock XF (ax0044XF)
YES Cells briefly expanded before inducing differentiation to achieve <90% pure cerebral cortical neurons with minimal cell body clumping. Protocol 3: Synchronous differentiation of Neural Stem Cells (Prior expansion)

Culturing Neural Cells in 96-well Format (High-density)

3 - For culturing neural cells long-term at high-density (up to 200,000 cells/cm2)
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ReagentsXeno-free​Description​Protocol
​- SureBond-XF (ax0041XF)
Neural Maintenance-XF (ax0032-500)
Neural Differentiation-XF Medium (ax0034-125)
Neural Expansion-XF (ax0030-500) 
- Plating-XF Medium (ax0033)
​YesFor culturing neural cells in 96-well format, either as neurons or as mixed populations, long-term and
at high-density
Protocol 5: Culturing Neural cells in 96-well format (High-density)
 

NSCs were plated on Sure Bond-XF (ax0041XF) coating at 95000 cells/cm2 and synchronously differentiated using Axol Neural Differentiation-XF Medium (ax0034-125). Cultured maintained in Axol Neural Maintenance-XF Medium (ax0032-500). Image taken at 21 DIV. Image inverted to highlight neurite processes.  

NSCs were plated on Sure Bond-XF (ax0041XF) coating at 187500 cells/cm2 and synchronously differentiated using Axol Neural Differentiation-XF Medium (ax0034-125). Cultured maintained in Axol Neural Maintenance-XF Medium (ax0032-500). Image taken at 21 DIV.  Image inverted to highlight neurite processes.  

CLICK HERE for high resolution image                                                     CLICK HERE for high resolution image                           

Culturing Neural Cells in 96-well Format (low-density)

4 - For culturing neural cells long-term at low-density (down to 10,000 cells/cm2)
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Reagents Xeno-free ​Description ​Protocol
​- Sure Bond+ (ax0041+)
Neural Maintenance-XF (ax0032-500)
Neural Differentiation-XF Medium (ax0034-125)
Neural Expansion-XF (ax0030-500)
-Plating-XF Medium
​No For culturing neural cells in 96-well format, either as neurons or as mixed populations, long-term and
at low-density
Protocol 6: Culturing Neural cells in 96-well plate format (low density)
 

Culturing Neural Cells on Glass

5- For culturing neural cells on glass surfaces such as multi-electrode arrays or microfluidic devices
Odoo CMS - a big picture
 

ReagentsXeno-free​Description​Protocol
​- Sure Bond+ (ax0041+)
Neural Maintenance-XF (ax0032-500)
Neural Differentiation-XF Medium (ax0034-125)
- Plating-XF Medium (ax0033)
​NoFor culturing neural cells on glass formats, either as neurons or as mixed populations, long-term.Protocol 7: Culturing Neural Cells on Glass 

TO FIND OUT MORE

Contact: Zoe Allen