Human iPSC-Derived Cerebral Cortical Neurons (Plated)

iPSC-derived cerebral cortical neurons

Axol Human iPSC-Derived Cerebral Cortical Neurons express typical markers of cerebral cortical neurons, such as TUJ1, TBR1, CTIP2, BRN2 and CUX1. They are electrically active and can form functional synapses and neuronal networks in vitro.

Axol supplies pre-plated neurons that have been synchronously differentiated from neural stem cells using our fully defined, neuronal differentiation method. Using our specialized coating reagents and Neural Maintenance-XF Medium, the iPSC-derived neurons can be maintained in culture long-term. 

Our expert scientists perform the neuronal differentiation and supply neurons in 96-well plate format. The plated neurons are  assay-ready saving you time and resources. The iPSC-derived neurons are shipped live so the neurons are not stressed by cryopreservation. Please inquire for custom plating requests or see the product pages below for further information.  


Plated iPSC-derived cerebral cortical neurons from healthy donors

Cat. No. Product Name Starting Material Quantity Price
ax0025 Plated Human iPSC-Derived Cerebral Cortical Neurons (Male) Cord Blood CD34+ Cells 96-well plate $2400
ax0026 Plated Human iPSC-Derived Cerebral Cortical Neurons (Female) Cord Blood CD34+ Cells 96-well plate $2400
ax0028 Plated Human iPSC-Derived Cerebral Cortical Neurons (Male) Fibroblasts (74 Yr Donor) 96-well plate $2400
ax0029 Plated Human iPSC-Derived Cerebral Cortical Neurons (Female) Fibroblasts (64 Yr Donor) 96-well plate $2400

Plated iPSC-derived cerebral cortical neurons from patient donors

Cat. No. Product Name Starting Material Quantity Price
ax0121 Plated Human iPSC-Derived Cerebral Cortical Neurons - Alzheimer's Disease Patient (APOE4 HOM) Fibroblasts (87 Yr Female) 96-well plate $2400
ax0122 Plated Human iPSC-Derived Cerebral Cortical Neurons - Alzheimer's Disease Patient (PSEN1 L286V) Fibroblasts (38 Yr Female) 96-well plate $2400
ax0123 Plated Human iPSC-Derived Cerebral Cortical Neurons - Alzheimer's Disease Patient (PSEN1 M146L) Fibroblasts (53 Yr Male) 96-well plate $2400
ax0124 Plated Human iPSC-Derived Cerebral Cortical Neurons - Alzheimer's Disease Patient (PSEN1 A246E) Fibroblasts (31 Yr Female) 96-well plate $2400
ax0125 Plated Human iPSC-Derived Cerebral Cortical Neurons - Alzheimer's Disease Patient (PSEN2 N141I) Fibroblasts (81 Yr Female) 96-well plate $2400
ax0222 Plated Human iPSC-Derived Cerebral Cortical Neurons - Huntington's Disease Patient (CAG:56) Fibroblasts (48 Yr Male) 96-well plate $2400
ax0421 Plated Human iPSC-Derived Cerebral Cortical Neurons - Epilepsy Patient Fibroblasts (5 Mo Female) 96-well plate $2400
ax1021 Plated Human iPSC-Derived Cerebral Cortical Neurons - Trisomy X Patient Fibroblasts (74 Yr Female) 96-well plate $2400

Plated neuron maintenance medium 

Cat. No. Product Name Product Description Quantity Price
ax0032-500 Neural Maintenance-XF Medium Xeno-free medium for maintenance of NSCs and neurons 500 mL $280

Characterization

Phase Contrast Images of Axol Human iPSC-Derived Cerebral Cortical Neurons

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Immunofluorescence

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Transcriptome

Gene expression profiling of Axol cerebral cortical neurons by microarray

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Up-regulated genes in cerebral cortical neurons

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Find the full dataset

The full primary datasets generated for our transcriptome analysis are available at NCBI GEO if you would like to perform your own analysis and look for expression of your gene of interest!


Neurite outgrowth

Live imaging of neurite outgrowth with IncuCyte and NeuroTrackTM by Essen Bioscience Ltd.

Competitor product comparision

Time-course comparison: N2a mouse neuroblastoma cell line, primary mouse cortical neurons (CTX), competitor's iPSC-derived neurons & Axol's cortical NSC-derived neurons

Live imaging data by Axol Bioscience

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Multi-electrode array (MEA)

MEA analysis of Axol iPSC-Derived Cerebral Cortical Neurons

Human iPSC-derived neurons may be effectively used for drug discovery. Our collaborators, Ikurou Suzuki and his team at Tohoku Institute of Technology in Japan used Alpha MED Scientific’s multi-electrod array (MEA) system to investigate the functional characteristics of human iPSC-derived neurons on their long-term spontaneous activity and drug responsiveness.

The results from this research, using Axol iPSC-Derived Cerebral Cortical Neurons and culture reagents, can be found in the posters and papers from our collaborators.

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Odawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016).

Drug effects on Axol iPSC-Derived Cerebral Cortical Neurons by Alpha MED Scientific

Axol Cerebral Cortical Neurons co-cultured with astrocyte on a MED Probe (MED-P515A, 134 days in vitro)
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64 channel oscilloscope data demonstrating spontaneous activity at 134 Days in vitro

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Spontaneous action potentials recorded from Axol Cerebral Cortical Neurons co-cultured with astrocytes (70 days in culture). The traces represent changes in the spontaneous action potentials detected by the same electrodes in response to drug application.

 
Read more about the performance of Axol cerebral cortical neurons using the MED64 platform

Application of Axol iPSC-Derived Cerebral Cortical Neurons on multi-electrode arrays for studying network electrophysiology by Ole Paulsen's Lab (University of Cambridge)

Spontaneous spiking activity in Axol iPSC-derived neuronal cultures

Complete MEA dataset by Dr Paul Charlesworth

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MEA studies of Axol iPSC-Derived Cerebral Cortical Neurons by Axion Biosystems

An activity map from the AxIS Software suite showing positions of spike activity for each well of the MEA

iPSC-Derived Cerebral Cortical Neuron morphology on Axion MEAs

Dedicated protocols for using Axol's neural stem cells on Axion MEAs and data generated with Axol's neurons

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3D cell culture

3D culture of Axol cerebral cortical neurons in a collagen gel

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TBR1   /   TUJ1

Left Panel: Top View
Top Right Panel: Bottom View
Bottom Panel: Side View

On culturing the cerebral cortical neurons  on top of a collagen gel, they formed a uniform static layer of cell bodies. Neurites projected out of these cells and grew downwards, creating a network of interconnected neurites. The results demonstrate that the collagen matrix was suitable for culturing cerebral cortical neurons to achieve neurite outgrowth, although little neuronal migration was observed. 

Find out more about 3D culture of Axol neural cells


Citations

Product citations