Drug Effects on Axol Cerebral Cortical Neurons (hCCNs)
- Axol Human Cerebral Cortical Neurons (hyCCNs) were co-cultured with astrocytes and their extracellular physiology properties were characterized using Alpha Med Scientific's MED64 microelectrode array platform.
- The image to the left depicts the cells after 56 days in co-culture.
Raw data acquired from all 64 electrodes (Acquisition bandwidth: 100Hz-10kHz) before drug administration (left panel) and the administration of 10 μM Bicuculline (right panel)
Spontaneous action potentials recorded at electrode 34 (69 days in culture). The traces represent changes in the spontaneous action potentials detected by the same electrode.
The trace at the top represent spontaneous neuronal spikes detected before drug administrations. Spike frequency increased in response to Bicuculline (10 μM). Activity was suppressed by the administartion of D(-)-2-Amino-5-phosphonovaleric Acid (AP5) (50 μM) and 6-cyano-7 nitroquinoxaline-2,30dione (CNQX) (30 μM) respectively. Spike frequency decreased in a subtle way by L-Glutamic acid (20 μM).
Changes of total number of spikes with drug administration.
- The results demonstrated that electrical activity of Axol Human Cerebral Cortical Neurons could be detected using the MED64 platform both before, during and after drug testing. The high-sensitivity of this method allowed the detection of signal changes due to drug effects.
- The MED64’s high-sensitivity enables detecting even small signals and/or subtle changes of drug effects from Axol Human Cerebral Cortical Neurons.
Many thanks to Alpha Med Scientific for their help in generating the above data using their MED64 system. Visit www.med64.com for more information about the MED64 platform.