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Primary Human Hepatocytes

Uncultured liver cells and expanded primary hepatocytes
primary hepatocytes

Primary
hepatocytes

Uncultured human hepatocytes & purified hepatic stellate, Kupffer & intra-hepatic biliary epithelial cells.
expanded primary hepatocytes

Assay-Ready Expanded primary hepatocytes

Expanded primary hepatocytes ready for CYP induction, metabolism and genotoxicity studies.
liver sinusoidal endothelial cells

ARE liver sinusoidal endothelial cells 

Assay-ready expanded liver sinusoidal endothelial cells and optimized culture medium. 

Primary human hepatocytes

Cryopreserved, uncultured primary human hepatocytes isolated from liver tissue. Our primary hepatocytes have been validated for CYP induction. Data on the fold induction of CYP1A2, CYP2B6 and CYP3A4 are generated for each lot of cells and is stated in the Certificate of Analysis for each order. They have post-thaw viability and plateability of >70%. We also offer purified hepatic stellate cells, intra-hepatic biliary epithelial cells and cryopreserved human Kupffer cells. 

Cat. No. Product Name Quantity
ax3750-1 Primary Human Hepatocytes - Non-Characterised 
>3 million cells/vial
ax37501-1 Primary Human Hepatocytes - Characterised 
>3 million cells/vial
ax3751-1 Primary Hepatocyte Plating Medium  250 mL
ax3752-1 Primary Hepatocyte Maintenance Medium  250 mL
ax3754-1 Primary Hepatocyte Thaw Medium  100 mL
ax3755 Primary Human Kupffer Cells  1 million cells
ax3756 Human Kupffer Cell Plating Medium  250 mL
ax3757 Human Kupffer Cell Maintenance Medium  250 mL
ax3758 Primary Human Kupffer Cell Starter Kit  1 vial of ax3755 Primary Kupffer Cells (1 million cells), 1 bottle of ax3756 Primary Kupffer Cell Plating Medium (250 mL),
1 bottle of ax3757 Primary Kupffer Cell Maintenance Medium (250 mL)
and 5 Type I Collagen-coated Multi-well Plates (96-well Plates) ax3799

ax37611-1 Human Hepatic Stellate Cell Growth Medium  250 mL
ax3760-1 Primary Human Hepatic Stellate Cells  100,000 cells *Lot dependent. Please inquire for latest lot quantity and price*
ax3761-1 Human Hepatic Stellate Cell Growth Medium  500 mL
ax3765-1 Human Intrahepatic Biliary Epithelial Cells  500,000 cells *Lot dependent. Please inquire for latest lot quantity and price*
ax3766-1 Intra-Hepatic Biliary Epithelial Cell Culture Medium  500 mL

Assay-Ready Expanded (ARE) human hepatocytes

Assay-Ready Expanded (ARE) hepatocytes are cryopreserved human hepatocytes that are easy to plate and ready to use for a wide range of applications including hepatic metabolism, compound uptake, CYP induction/inhibition, hepatotoxicity, genotoxicity and 3D co-culture studies. See the Application Data here! ARE Hepatocytes express CYP enzymes, are metabolically functional, are polarized and can be infected by the Hepatitis C virus.

ARE Hepatocytes are primary human hepatocytes that have been expanded via activation of the Oncostatin M pathway. Large batch sizes of hepatocytes from the same donor are available on request, providing a reliable and consistent source of primary hepatocytes. ARE Hepatocytes should be used directly for endpoint assays and cannot be expanded further since de-differentiation may occur.

Our expanded primary hepatocytes have high viability and >90% plateability when using ARE Hepatocyte Thawing Medium & Maintenance Medium.

For genotoxicity applications, we have validated hepatocytes (ax3703) that can be used for micronucleus assays when grown in ARE Hepatocyte Genotoxicity Assay Medium. This medium permits hepatocyte cell division allowing micronucleus formation to be observed when hepatocytes are exposed to genotoxic compounds.

Cat. No. Product Name Quantity
ax3701 Assay-Ready Expanded (ARE) Hepatocytes 
5 million cells 
ax3702 Assay-Ready Expanded (ARE) Hepatocytes, CYP2D6 Overexpressing 
5 million cells
ax3703 Assay-Ready Expanded (ARE) Hepatocytes, Genotoxicity Assay Validated 
1 million cells
ax3704 ARE Hepatocyte Starter Kit 
1 kit (5 million cells) 
ax3705 ARE Hepatocyte Thawing Medium 
50 mL
ax3710 ARE Hepatocyte Maintenance Medium 
500 mL
ax3715 ARE Hepatocyte Genotoxicity Assay Medium 
500 mL

Human liver sinusoidal endothelial cells

Assay-Ready Expanded (ARE) liver sinusoidal endothelial cells are easy to plate, cryopreserved liver endothelial cells that have been previously expanded in vitro. Our liver sinusoidal endothelial cells express CD31+ and Mannose receptor and are validated for uptake of Low Density Lipoprotein (LDL). Large batch sizes of liver endothelial cells from the same donor are available on request, ensuring consistency from preliminary experiments to high-throughput screening. ARE liver sinusoidal endothelial cells can be passaged twice in our optimized liver sinusoidal endothelial cell culture medium. The medium contains recombinant growth factors tailored for promoting liver endothelial cell viability and growth. 

Cat. No. Product Name Quantity
ax3778-1 Sinusoidal Endothelial Growth Medium 
500 mL
ax3777-1 Primary Human Liver Sinusoidal Endothelial Cells 
>500.000 cells/vial *Lot dependent. Please inquire for latest lot quantity and price*
ax3720 Assay-Ready Expanded (ARE) Liver Sinusoidal Endothelial Cells 
5 million cells
ax3721 ARE Liver Sinusoidal Endothelial Cell Culture Medium 
100 mL
ax3722 ARE Liver Sinusoidal Endothelial Starter Kit 
Kit including: ARE Liver Sinusoidal Endothelial Cells (ax3720; 5 million cells),
ARE Liver Sinusoidal Endothelial Cell Culture Medium (ax3721; 100 mL)
and ax3799 Type I Collagen-coated Plates (96-well plates, Pack of 5)


Application Notes

ARE hepatocyte and liver sinusoidal endothelial cell application data: metabolism, CYP induction/inhibition, hepatotoxicity, genotoxicity and co-culture data


Custom cell & tissue sourcing

Can't find what you're looking for? Our custom cell & tissue sourcing service can help you find primary human cells or tissue
from healthy and patient donors. Simply tell us your specifications and we will endeavour to meet your requirements.
Custom cell sourcing