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Primary Human Hepatocytes

Uncultured liver cells and expanded primary hepatocytes

Primary
hepatocytes

Uncultured human hepatocytes & purified hepatic stellate, Kupffer & intra-hepatic biliary epithelial cells.

Assay-Ready Expanded primary hepatocytes

Expanded primary hepatocytes ready for CYP induction, metabolism and genotoxicity studies.

ARE liver sinusoidal endothelial cells 

Assay-ready expanded liver sinusoidal endothelial cells and optimized culture medium. 

Hepatic
organoids

Adult liver stem cells cultured within an extracellular matrix self-assemble to form 3D organoids.

Primary human hepatocytes

Cryopreserved, uncultured primary human hepatocytes isolated from liver tissue. Our primary hepatocytes have been validated for CYP induction. Data on the fold induction of CYP1A2, CYP2B6 and CYP3A4 are generated for each lot of cells and is stated in the Certificate of Analysis for each order. They have post-thaw viability and plateability of >70%. We also offer purified hepatic stellate cells, intra-hepatic biliary epithelial cells and cryopreserved human Kupffer cells. 

Cat. No. Product Name Quantity
ax3750 Primary Hepatocytes 
>1 million cells*
*Lot dependent
ax3751 Primary Hepatocyte Plating Medium  250 mL
ax3752 Primary Hepatocyte Maintenance Medium  250 mL
ax3753 Primary Hepatocyte Starter Kit 
1 kit (5 million cells*)
ax3755 Cryopreserved Human Kupffer Cells  1 million cells
ax3756 Human Kupffer Cell Plating Medium  250 mL
ax3757 Human Kupffer Cell Maintenance Medium  250 mL
ax3758 Human Kupffer Cell Starter Kit  1 kit (1 million cells)
ax3760 Cryopreserved Human Hepatic Stellate Cells  100,000 cells
ax3761 Hepatic Stellate Cell Culture Medium  250 mL
ax3762 Hepatic Stellate Cell Starter Kit  1 kit (100,000 cells)
ax3765 Intra-Hepatic Biliary Epithelial Cells  500,000 cells
ax3766 Intra-Hepatic Biliary Epithelial Cell Culture Medium  500 mL
ax3767 Intra-Hepatic Biliary Epithelial Cell Starter Kit  1 kit (500,000 cells)

Assay-Ready Expanded (ARE) human hepatocytes

Assay-Ready Expanded (ARE) hepatocytes are cryopreserved human hepatocytes that are easy to plate and ready to use for a wide range of applications including hepatic metabolism, compound uptake, CYP induction/inhibition, hepatotoxicity, genotoxicity and 3D co-culture studies. See the Application Data here! ARE Hepatocytes express CYP enzymes, are metabolically functional, are polarized and can be infected by the Hepatitis C virus.

ARE Hepatocytes are primary human hepatocytes that have been expanded via activation of the Oncostatin M pathway. Large batch sizes of hepatocytes from the same donor are available on request, providing a reliable and consistent source of primary hepatocytes. ARE Hepatocytes should be used directly for endpoint assays and cannot be expanded further since de-differentiation may occur.

Our expanded primary hepatocytes have high viability and >90% plateability when using ARE Hepatocyte Thawing Medium & Maintenance Medium.

For genotoxicity applications, we have validated hepatocytes (ax3703) that can be used for micronucleus assays when grown in ARE Hepatocyte Genotoxicity Assay Medium. This medium permits hepatocyte cell division allowing micronucleus formation to be observed when hepatocytes are exposed to genotoxic compounds.

Cat. No. Product Name Quantity
ax3701 Assay-Ready Expanded (ARE) Hepatocytes 
5 million cells 
ax3702 Assay-Ready Expanded (ARE) Hepatocytes, CYP2D6 Overexpressing 
5 million cells
ax3703 Assay-Ready Expanded (ARE) Hepatocytes, Genotoxicity Assay Validated 
1 million cells
ax3704 ARE Hepatocyte Starter Kit 
1 kit (5 million cells) 
ax3705 ARE Hepatocyte Thawing Medium 
50 mL
ax3710 ARE Hepatocyte Maintenance Medium 
500 mL
ax3715 ARE Hepatocyte Genotoxicity Assay Medium 
500 mL

Human liver sinusoidal endothelial cells

Assay-Ready Expanded (ARE) liver sinusoidal endothelial cells are easy to plate, cryopreserved liver endothelial cells that have been previously expanded in vitro. Our liver sinusoidal endothelial cells express CD31+ and Mannose receptor and are validated for uptake of Low Density Lipoprotein (LDL). Large batch sizes of liver endothelial cells from the same donor are available on request, ensuring consistency from preliminary experiments to high-throughput screening. ARE liver sinusoidal endothelial cells can be passaged twice in our optimized liver sinusoidal endothelial cell culture medium. The medium contains recombinant growth factors tailored for promoting liver endothelial cell viability and growth. 

Cat. No. Product Name Quantity
ax3720 Assay-Ready Expanded (ARE) Liver Sinusoidal Endothelial Cells 
5 million cells
ax3721 ARE Liver Sinusoidal Endothelial Cell Culture Medium 
100 mL
ax3722 ARE Liver Sinusoidal Endothelial Starter Kit 
1 kit (5 million cells)

Human hepatic organoids

Derived from stem cells, the organoids are self-renewing and proliferative, allowing further expansion to meet the needs of your study. Their physiological responses are retained from their tissue of origin which provides relative toxicological data. Applicable in multiple bioscience applications these organoids are a valuable alternative to hepatic cell lines and primary hepatocytes.

Cat. No. Product Name Quantity
ax41163 Human Hepatic Organoids See product page

Application Notes

ARE hepatocyte and liver sinusoidal endothelial cell application data: metabolism, CYP induction/inhibition, hepatotoxicity, genotoxicity and co-culture data


Custom cell & tissue sourcing

Can't find what you're looking for? Our custom cell & tissue sourcing service can help you find primary human cells or tissue
from healthy and patient donors. Simply tell us your specifications and we will endeavour to meet your requirements.
Custom cell sourcing