Huntington's Disease Neural Stem Cells
iPSC-derived Huntington's Disease Neural Stem Cells
We have applied our innovative technology to develop human neural stem cells directly differentiated from Huntington’s Disease iPS cells. Our cells allow Huntington's Disease researchers to build their own human cellular models in a disease-relevant genetic and tissue context.
Our ready-to-use kits allow you to concentrate on your experiments rather than spending valuable time and resources generating Huntington's Disease neural progenitors in-house. As part of the HD NSC kit, you will receive Plating-XF Medium in addition to your vial of human neural stem cells to maximize cell viability.
HD NSCs are ready made and easy to differentiate. They are capable of differentiating into a spectrum of cortical excitatory and inhibitory neurons and remain viable in culture for months, facilitating reproducible and long-term studies.
Whether you want to study Huntington's Disease pathogenesis at the cellular level, perform neurotoxicity testing or pre-clinical drug screening, we believe that HD NSCs, alongside our optimized culture reagents, provide the perfect culture system for your research success.
Axol Human iPSC-derived Cardiomyocytes Spontaneously Beat in The Dish!
Axol Human iPSC-derived Cardiomyocytes Spontaneously Beat in 2D Culture
In the video below you can see how the cardiomyocytes begin to spontaneously beat after just 7 days in culture following thawing!
Axol Human iPSC-derived Cardiomyocytes Can Form Cardiospheres
Axol cardiomyocytes form spontaneously beating spheroids when seeded in hanging drops. A cardiosphere at 10 days in culture can be seen in the video below. (Kindly provided by Christian Zuppinger, University of Bern)
Protein Expression in Axol iPSC-derived Cardiomyocytes
Axol human iPSC-derived cardiomyocytes were cultured on vessels coated with Sure BondXF. The cells were cultured in iPSC Cardiomyocyte Maintenance Medium prior to being fixed and stained for the markers Sarcomeric Alpha Actinin and Cardiac Troponin. The cells were counterstained using DAPI to allow visualization of nuclei.
In a study by Christian Zuppinger at the University of Bern, Axol's iPSC-derived cardiomyocytes were cultured for 10 days then fixed and stained for a number of markers. In the image to the right, the cells were stained for the sarcomeric marker myomesin, which labels the M-line of sarcomeres in all species (green) and all actin (red). The cells were counterstained using DAPI to allow visualization of nuclei. The results demonstrate that there is good sarcomere differentiation in these cells and the majority of cells are positive for muscle proteins.
In the images below, the 10 day cultured iPSC-derived cardiomyocytes were fixed and stained for the gap junction protein connexin-43 (green) and all actin (red). Connexin-43 was found to be expressed by all cells as shown in the representative images.
Many thanks to Christian Zuppinger for sharing both his expertise and the data he generated using Axol's human iPSC-derived cardiomyocytes.