Human iPSC-Derived Cardiomyocytes

iPSC-derived cardiomyocytes

Our human iPSC-derived cardiomyocytes allow scientists to build their own human cellular models to accelerate their research. The use of iPSC-derived cardiomyocytes as an in vitro research model provides a major advantage over primary cells in that they provide a continuous source of cardiomyocytes from the same genetic background for use in multiple experiments. Our human cardiomyocytes derived from iPS cells are ideal for use in cardiotoxicity testing, drug screening, drug validation as well as metabolism studies and electrophysiology applications.

Once thawed, our cardiomyocytes will soon begin to spontaneously beat in vitro and are characterized by the expression of cardiac-specific markers, including cardiac troponin (see the data below). We also provide a fully-defined culture medium and coating reagents optimized for use with the cardiomyocytes for maximal recovery and viability for experiment assays. 

Consistent
results

Reliably reproduce test results.

Serum-free
system

Maintain pure cultures for long-term experiments.

Regular
beating rate

No need to pre-plate.

Efficient
plating

Validated on Fibronectin, Vitronectin, and Matrigel substrates. 


Human iPSC-derived ventricular cardiomyocytes

Cat. No. Product Name Quantity Price
ax2502 Human iPSC-Derived Ventricular Cardiomyocytes (Female) 1 million cells $400 (Pre-order now)
ax2505 Human iPSC-Derived Ventricular Cardiomyocytes (Male) 1 million cells $400
ax2520 Human iPSC-Derived Ventricular Cardiomyocytes (Male) 1 million cells $400
ax2500 Human iPSC-Derived Ventricular Cardiomyocyte Kit (Male) 1 kit (1 million cells) $880

Cardiomyocyte culture media and reagents

Cat. No. Product Name Quantity Price
ax2530-500 Cardiomyocyte Maintenance Medium 500 mL $280
ax0044 Unlock 25 mL $96
ax0049 Fibronectin Coating Solution 1 mL $240

Human iPSC-derived atrial cardiomyocytes (in development)

To register your interest in Axol Human iPSC-Derived Atrial Cardiomyocytes or for further information, please fill in the form below and we will be in touch.




Characterization

Marker expression 

Axol Human iPSC-Derived Ventricular Cardiomyocytes were cultured in Cardiomyocyte Maintenance Medium prior to being fixed and stained for the markers Sarcomeric Alpha Actinin and Cardiac Troponin. The cells were counterstained using DAPI to allow visualization of nuclei.  

iPSC CMs Sarcomeric Alpha Actinin and DAPI
Sarcomeric Alpha Actinin and DAPI
Cardiac Troponin T and DAPI
Cardiac Troponin T and DAPI

In a study by Christian Zuppinger at the University of Bern, Axol ventricular cardiomyocytes were cultured for 10 days then fixed and stained for a number of markers.  In the image to the right, the cardiomyocytes were stained for the sarcomeric marker myomesin, which labels the M-line of sarcomeres in all species and all actin. The cells were counterstained using DAPI to allow visualization of nuclei. The results demonstrate that there is good sarcomere differentiation in these cells and the majority of cells are positive for muscle proteins.

Odoo image and text block

In a later study, the ventricular cardiomyocytes were cultured for two weeks during which they formed a closed carpet in 2D culture. The image on the left shows the expression of the beta-myosin heavy chain and all actin  in the cardiomyocytes. The cells were also counterstained with DAPI to visualize the nuclei.

'This batch of cardiomyocytes has exceeded expectations.'

Christian Zuppinger, University of Bern. December 2015.

Many thanks to Christian Zuppinger for sharing both his expertise and the data he generated using Axol Human iPSC-Derived Ventricular Cardiomyocytes.

In the images below, ventricular cardiomyocytes were fixed after 10 days in culture and stained for the gap junction protein connexin-43 and all actin .

Connexin-43 was found to be expressed by all cells as shown in the representative images.

Signaling and stress response
In collaboration with Christian Zuppinger - University of Bern

Telethonin is a sarcomeric titin-binding protein that may also play a role in signaling and stress-response.

Ankyrin repeat domain 1 (ANKRD1) is a cytoskeleton linker protein that acts as a stress-sensor in cardiac muscle. 

Ventricular cardiomyocytes were coimmunostained for Telethonin,  Actin and  DAPI  counterstain .
Ventricular cardiomyocytes were coimmunostained for Ankyrin repeat domain 1 (ANKRD1), Actin and DAPI counterstain. 

Beating

In this video you can see how Axol Human iPSC-Derived Ventricular Cardiomyocytes begin to spontaneously beat after just 7 days in culture following thawing!

 
 

Cardiospheres

Axol Human iPSC-Derived Ventricular Cardiomyocytes form spontaneously beating spheroids when seeded in hanging drops. A cardiosphere after 10 days in culture can be seen in this video. (Kindly provided by Christian Zuppinger, University of Bern)


Calcium imaging

Axol Human iPSC-Derived Ventricular Cardiomyocytes can be used in calcium imaging assays as shown in this video. The data were provided by Dr Frances Brook and Dr Yu-Fen Chang, Oxford University. The cardiomyocytes were plated and cultured for seven days in Axol Cardiomyocyte Maintenance Medium, and then replated on Fibronectin/Gelatin (0.5% / 0.1%) coated plates for final analysis.


Modeling arrhythmias

Data provided by Dr J. Maynes University of Toronto

Axol Human iPSC-Derived Ventricular Cardiomyocytes were treated with doxorubicin (control, 500 nM, 100 nM and 50 nM).
There was a dose-dependent response at 13 h and 24 h post-treatment with doxorubicin. Arrhythmias and loss of beating amplitude were seen at lower doxorubicin concentration, whereas higher doxorubicin concentrations resulted in tachycardia and ultimately cell death.


Posters and citations

In vitro assays and screening platforms for exploring ventricular and atrial phenotypes in iPSC-derived cardiomyocytes

Serum-free human iPSC-derived cardiomyocytes for in vitrotesting

Effects of transient hypoxia/ischemia on inducible human pluripotent stem cell-derived cardiomyocytes

A drug discovery platform for the identification of novel infarct sparing agents for treatment of ischemic heart diseases


Application

Non-invasive impedance monitoring of contractility in Axol Human iPSC-Derived Cardiomyocytes

Application note

Non-invasive impedance monitoring of contractility in Axol Human iPSC-Derived Cardiomyocytes.

by Dr Jason Gill, Durham University (UK)


Disease Modeling

Have your own patient samples? Let us help generate your disease models. We provide custom iPSC Reprogramming, Differentiation and Genome Editing services to save your time and resources.

Cardiomyocyte Cell Guide

An overview of iPSC-derived cardiomyocytes and their applications.