Mixed Population of Neural Cells

Overview

With this system, Neural Stem Cell (NSC) populations can be differentiated into mixed populations of neurons, astrocytes and oligodendrocytes.  All reagents are fully-defined, animal component-free and have been developed together to ensure optimal consistency for your research. Built to be modern, our media formulations incorporate a number of tools validated within the neuroscience field to improve key research areas including electrophysiology, neurite outgrowth/branching and long-term culturing.

Low-density cultures

Mixed populations of neural cells

Long-term culturing capacity (>50 days)

Maximal adherence.  No cell body clumping

 

Scroll down to discover application workflows, protocols & images

 
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Workflows

Spontaneous Differentiation

1 - Obtain a mixed population of neural cells including astrocytes, oligodendrocytes and neurons. 
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ReagentsXeno-free​Description​Protocol
​- SureBond-XF (ax0041XF)
- Neural Expansion-XF (ax0030-500)
- Unlock-XF (ax0044XF)
- Neural Maintenance-XF (ax0032-500)
-Plating-XF Medium (ax0033)		​Yes​Obtain a mixed population of neural cells including cerebral cortical neurons, oligodendrocytes and astrocytes​Protocol 4: Spontaneous differentiation of Neural Stem Cells
 

Culturing Neural Cells in 96-well Format (High-density)

2 - For culturing of neural cells long-term at high-density (up to 200000 cells/cm2)
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ReagentsXeno-free​Description​Protocol
​- SureBond-XF (ax0041XF)
- Neural Maintenance-XF (ax0032-500)
- Neural Differentiation-XF Medium (ax0034-125)
- Neural Expansion-XF (ax0030-500)
-Plating-XF Medium (ax0033)		​YesFor culturing neural cells in 96-well format, either as neurons or as mixed populations, long-term and
at high-density		​Protocol ​5: Culturing Neural cells in 96-well format (High-density)
 

NSCs were plated on Sure Bond-XF (ax0041XF) coating at 95000 cells/cm2 and synchronously differentiated using Axol Neural Differentiation-XF Medium (ax0034-125). Cultured maintained in Axol Neural Maintenance-XF Medium (ax0032-500). Image taken at 21 DIV. Image inverted to highlight neurite processes.  

NSCs were plated on Sure Bond-XF (ax0041XF) coating at 187500 cells/cm2 and synchronously differentiated using Axol Neural Differentiation-XF Medium (ax0034-125). Cultured maintained in Axol Neural Maintenance-XF Medium (ax0032-500). Image taken at 21 DIV.  Image inverted to highlight neurite processes.  

CLICK HERE for high resolution image                                                                           CLICK HERE for high resolution image      

Culturing Neural Cells in 96-well Format (Low-density)

3 - For culturing of neural cells long-term at low-density (up to 10000 cells/cm2)
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Reagents Xeno-free ​Description ​Protocol
​- Sure Bond+ (ax0041+)
- Neural Maintenance-XF (ax0032-500)
- Neural Differentiation-XF Medium (ax0034-125)
- Neural Expansion-XF (ax0030-500)
- Plating XF Medium (ax0033)		 ​No For culturing neural cells in 96-well format, either as neurons or as mixed populations, long-term and
at low-density		 Protocol  ​ 6: Culturing Neural cells in 96-well plate format (low density)

Culturing Neural Cells on Glass

4- For culturing of neural cells on glass surfaces such as multi-electrode arrays and microfluidic devices
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Reagents Xeno-free ​Description ​Protocol
​- Sure Bond+ (ax0041+)
- Neural Maintenance-XF (ax0032-500)
- Neural Differentiation-XF Medium (ax0034-125)
- Plating-XF Medium (ax0033)		 ​No For culturing neural cells on glass formats, either as neurons or as mixed populations, long-term.  ​ Protocol 7: Culturing Neural cells on glass

TO FIND OUT MORE

Contact: Dr. Zoe Allen