In order to examine synaptic connectivity and action potential potential propagation using Axol's neural cells, we collaborated with Ole Paulsen's lab at the University of Cambridge. MEA technology was used for our assays as it provides a label-free, non-invasive operation that avoids perturbation of natural cell function. Another advantage of this technology is that multiple recording sites are recorded simultaneously, facilitating the speed of the experiments. In the experiments, the MEA-1060 system from MultiChannel Systems is used.
Spontaneous Spiking Activity Observed in Axol Cortical Neural Stem Cell-derived Neuronal Cultures
NSCs on MEA dish
600 and 35 s raster plots
Spike Rate Histogram
Spike Overlay (10 consecutive events) from the Same Channel
Long Term Display
Development of Network Activity
Network Activity Week 4
Network Activity Week 5
Network Activity Week 6: Emergence of Burst Firing. At 6 weeks, the first evidence of array wide burst firing is seen. (Burst denoted by blue lines, calculated using critical time interval algorithm).