Human iPSC-Derived Neural Stem Cells

Neural stem cells - cerebral cortex

Axol's cerebral cortical neural stem cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions.

Axol Human iPSC-Derived Neural Stem Cells  express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6 and FOXG1. They spontaneously form polarized neural rosette structures when cultured as a monolayer. Additionally, Axol's NSCs can generate cortical neurons that are electrically active and have the ability to form functional synapses and neural circuits in vitro.


Human iPSC-derived neural stem cells from healthy donors

Cat. No. Product Name Starting Material Quantity Price
ax0011 Human iPSC-Derived Neural Stem Cells (Male) Cord Blood CD34+ Cells 1.5 million cells & Neural Plating-XF Medium $560
ax0015 Human iPSC-Derived Neural Stem Cells (Male) Cord Blood CD34+ Cells 1.5 million cells & Neural Plating-XF Medium $560
ax0016 Human iPSC-Derived Neural Stem Cells (Female) Cord Blood CD34+ Cells 1.5 million cells & Neural Plating-XF Medium $560
ax0018 Human iPSC-Derived Neural Stem Cells (Male) Fibroblasts (74 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $560
ax0019 Human iPSC-Derived Neural Stem Cells (Female) Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $560

Human iPSC-derived neural stem cells from patient donors

Cat. No. Product Name Starting Material Quantity Price
ax0111 Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (APOE4 HOM) Fibroblasts (87 Yr Female) 1.5 million cells & Neural Plating-XF Medium $560
ax0112 Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 L286V) Fibroblasts (38 Yr Female) 1.5 million cells & Neural Plating-XF Medium $560
ax0113 Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 M146L) Fibroblasts (53 Yr Male) 1.5 million cells & Neural Plating-XF Medium $560
ax0114 Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN1 A246E) Fibroblasts (31 Yr Female) 1.5 million cells & Neural Plating-XF Medium $560
ax0115 Human iPSC-Derived Neural Stem Cells - Alzheimer's Disease Patient (PSEN2 N141I) Fibroblasts (81 Yr Female) 1.5 million cells & Neural Plating-XF Medium $560
ax0211 Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient Fibroblasts (48 Yr Female) 1.5 million cells & Neural Plating-XF Medium $560
ax0411 Human iPSC-Derived Neural Stem Cells - Epilepsy Patient 
Fibroblasts (5 Mo Female) 1.5 million cells & Neural Plating-XF Medium $560
ax1001 Human iPSC-Derived Neural Stem Cells - Trisomy X Patient 
Fibroblasts (74 Yr Female) 1.5 million cells & Neural Plating-XF Medium $560

axolGEM (genetically edited model) human iPSC-derived neural stem cells

Cat. No. Product Name Starting Material Quantity Price
ax0019 Human iPSC-Derived Neural Stem Cells (Female) Isogenic Control Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $560
ax0310 axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HOM  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0311 axolGEM iPSC-Derived Neural Stem Cells LRRK2 G2019S HET  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0320 axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HOM  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0321 axolGEM iPSC-Derived Neural Stem Cells MAPT R406W HET  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0322 axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0323 axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HET  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0324 axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HOM  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280
ax0325 axolGEM iPSC-Derived Neural Stem Cells MAPT P301L HET  Fibroblasts (64 Yr Donor) 1.5 million cells & Neural Plating-XF Medium $1280

Neural cell culture media and reagents

Cat. No. Product Name Product Description Quantity Price
ax0101 Neural Stem Cell Media & Reagent Bundle - Expansion and Synchronous Differentiation (System A) Contains all of the neural media and reagents required to expand and synchronously differentiate Axol neural stem cells and maintain the differentiated neurons See Product Page $1920
ax0102 Neural Stem Cell Media & Reagent Bundle - Synchronous Differentiation (System B) Contains all of the neural media and reagents required to synchronously differentiate Axol neural stem cells and maintain the differentiated neurons See Product Page $920
ax0103 Neural Stem Cell Media & Reagent Bundle - Expansion and Spontaneous Differentiation (System C) Contains all of the neural media and reagents required to expand and spontaneously differentiate Axol neural stem cells and maintain the differentiated neurons and glial cells See Product Page $1760
ax0104 Neural Stem Cell Media & Reagent Bundle - Spontaneous Differentiation (System D) Contains all of the neural media and reagents required to spontaneously differentiate Axol neural stem cells and maintain the differentiated neurons and glial cells See Product Page $760
ax0030-500 Neural Expansion-XF Medium Xeno-free growth medium for expansion of NSCs 500 mL $280
ax0034-125 Neural Differentiation-XF Medium Xeno-free medium for synchronous differentiation of NSCs to neurons 125 mL $240
ax0032-500 Neural Maintenance-XF Medium Xeno-free medium for maintenance of NSCs and neurons 500 mL $280
ax0033 Neural Plating-XF Medium Xeno-free medium for plating NSCs after thawing and passaging 30 mL $80
ax0041 SureBond Coating reagent needed when passaging of NSCs is required 3 x 120 μL vials $200
ax0041+/ax0052 SureBond+ReadySet Coating reagent needed for endpoint assays on glass 3 x 120 μL vials & 2 x 10 mL vials $240
ax0041XF/ax0053 SureBond-XF Xeno-free coating reagent needed for endpoint assays on plastic 1 mL $400
ax0044 Unlock Gentle cell detachment solution to be used with SureBond 25 mL $96
ax0044XF/ax0054 Unlock-XF Xeno-free cell detachment solution to be used with SureBond-XF 100 mL $120
ax0047 Recombinant Human FGF2 Required for supplementation of Neural Expansion-XF Medium 100 μg $216
ax0047X/ax0048 Recombinant Human EGF Required for supplementation of Neural Expansion-XF Medium 100 μg $96
ax0051 ReadyFect Transfection reagent optimized for transfection of NSCs 40 μL $144

Characterization

Phase contrast

Axol iPSC-Derived Neural Stem Cells grown in Neural Expansion-XF Medium with the addition of EGF and FGF2.

Two hours after thawing.

Two days after thawing.

Six days after thawing.

Immunofluorescence


Transcriptome

Gene expression profiling of neural stem cells by microarray

Up-regulated RNA expression

Down-regulated RNA expression

The full primary data sets generated for our transcriptome analysis are available from Gene Expression Omnibus.

View the full dataset

Transfection

Transfection of cerebral cortical neural stem cells with ReadyFect

ReadyFect leads to 30-40% transfection efficiency. The image demonstrates transfection results using 3 µL of ReadyFect / 1 µg of a GFP-encoding vector (pVectOZ-GFP). GFP expression was evaluated two days post-transfection.


Neurite outgrowth

Live imaging of neurite outgrowth with IncuCyte and NeuroTrackTM by Essen Bioscience Ltd.

Competitor product comparision

Time-course comparison: N2a mouse neuroblastoma cell line, primary mouse cortical neurons (CTX), competitor's iPSC-derived neurons & Axol's cortical NSC-derived neurons

Live imaging data by Axol Bioscience

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Patch clamp

Whole cell patch clamp recordings by Ole Paulsen's Lab (University of Cambridge)

Electrophysiological characterization of Axol's iPSC-Derived Cerebral Cortical Neurons

Whole cell patch clamp recordings for characterizing neuronal electrical properties by Dr Paul Charlesworth

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Multi-electrode array (MEA)

MEA analysis in Axol iPSC-Derived Cerebral Cortical Neurons

Human iPSC-derived neurons may be effectively used for drug discovery. Our collaborators, Ikurou Suzuki and his team at Tohoku Institute of Technology in Japan used Alpha MED Scientific’s multi-electrod array (MEA) system to investigate the functional characteristics of human iPSC-derived neurons on their long-term spontaneous activity and drug responsiveness.

The results from this research, using Axol iPSC-Derived Cerebral Cortical Neurons and culture reagents, can be found in the posters and papers from our collaborators.

Odawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015).

Odawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016).

Application of Axol iPSC-Derived Cerebral Cortical Neurons on multi-electrode arrays for studying network electrophysiology by Ole Paulsen's Lab (University of Cambridge)

Spontaneous spiking activity in Axol iPSC-derived neuronal cultures

Complete MEA dataset by Dr Paul Charlesworth

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Multi-electrode array studies of Axol iPSC-Derived Cerebral Cortical Neurons by Axion Biosystems

An activity map from the AxIS Software suite showing positions of spike activity for each well of the MEA

iPSC-Derived Cerebral Cortical Neuron morphology on Axion MEAs

Dedicated protocols for using Axol's neural stem cells on Axion MEAs and data generated with Axol's neurons

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Transplantation

Transplantation of Axol iPSC-Derived Neural Stem Cells into neonatal rat cerebral cortex by Melissa Andrews' lab (University of St Andrews)

Coronal sections from adult rat cortex six weeks after transplantation of Axol iPSC-Derived NSCs. Image shows human-specific NCAM (red).

Transplantation of Axol-iPSC Derived NSCs into neonatal rat cerebral cortex by Dr Melissa Andrews

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Posters and citations

Posters

Next-generation neurological disease models: isogenic tools for investigating frontotemporal dementia, Alzheimer's and Parkinson's diseases

Functional phenotypic characterization of iPSC-neurons from Alzheimer’s disease patients carrying PS-1 mutation in drug screening and disease modeling

Characterization and potential applications of human iPSC-derived neural stem cells

Modelling neurological disease: in vitro gene editing and iPSC differentiation combine to create powerful new tools

Induction of plasticity phenomena in human induced pluripotent stem cell-derived cortical neurons

Functional maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture

Pharmacological responses in cultured human iPSC-derived cortical neurons using multi-electrode array

In vitro electrophysiological drug testing using human induced-pluripotent stem cells

Implementation of high throughput screening on iPS cells

Using iPSC-derived neural stem cells as a CNS model to study neuronal behaviour in development and neurodegernation

Modelling Alzheimer's disease: Development of a scalable, high-throughput-compatible assay to detect tau aggregates using iPSC-derived cortical neurons maintained in a 3D-culture format


Product citations