Cell Reprogramming

A complete suite of services

Our custom reprogramming and characterization services empower you to focus on your research interests and not use up your valuable time and resources generating cells.

Simply send us your donor cells (fibroblasts or peripheral blood mononuclear cells (PBMCs)) and we'll use cutting edge reprogramming technologies (non-integrating episomal or Sendai virus reprogramming) to produce induced pluripotent stem cells (iPSCs) within a short period of time (usually 2 to 3 months).

If you are struggling to find the right donor cells for reprogramming, we can also help you source fibroblasts and PBMCs with a wide range of donor backgrounds from our established network.

Once you receive the iPSCs from us, our dedicated specialists will share their expertise and provide ongoing support to help ease the adaptation of the cells into your experimental systems.

What we can do for you

Our reprogramming service includes:

Recovery, passaging, initial testing and banking of donor fibroblasts or PBMCs
Transfection of cells with non-integrating, zero-footprint systems: episomal plasmid vector or transduction with Cyto Tune-iPS 2.0 Sendai virus systems
A choice between a feeder-free and feeder-layer culture system
Analysis of pluripotency using immunocytochemistry and TaqMan hiPSC Scorecard Assay
Provision of more than one iPSC colony from a single donor

Additional 'add-on' services include:

Human pathogen testing
Short-Tandem Repeat (STR) profiling
qPCR analysis for pluripotency markers
Karyotyping (G-Banding)
Additional clones
Adaptation of iPSCs from a feeder layer to a feeder-free system
Additional ICC markers
Identification of cell surface markers by flow cytometry
Electrophysiological characterization via multi-electrode array (MEA)
iPS diffferentiation to multipe important cell lineages and supply of ready-to-use/cryopreserved cells
Genome editing and creation of isogenic cell lines with CRISPR-Cas9

Timeline

Our workflow

Axol's Stem Cell Reprogramming Service Timeline

Let us know

Do you have any questions about our cell reprogramming services?

Our dedicated Services team can help you. Our cell reprogramming services can be tailored to meet your experimental requirements, helping you to save time and resources. Please also dowmload our Statement of Use for further details.

Your opinion matters

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