Custom Reprogramming

Generation of iPSCs from human cells to meet your research needs

Deriving, maintaining and differentiating pluripotent stem cells is a time consuming and labor intensive process, especially when you are dealing with multiple lines. By using our cell reprogramming and characterization services, you can save hundreds of hours and thousands of dollars on tissue culture. We empower scientists to focus on their research interests and not consume valuable time and resources in the generation of cells prior to the experiments.  

Simply send us your donor cells and we will produce induced pluripotent stem (iPS) cells within a short period of time. If you couldn't find suitable donor samples, let us know. We are happy to source the cells for you.

Once you receive the derived iPS cells from us, our dedicated specialists will share their expertise and provide ongoing support to facilitate adaptation of the cells in your experimental systems. 

Contact us now to find more!

Not Just Reprogramming

Many researchers are interested in generating iPS cells for the purposes of disease modeling or regenerative medicine research. However, sourcing cell samples with required disease / healthy genetic background is often difficult. To make it easy for you, we can help to search for donor cell samples through our established network, or create disease-specific mutations / correct mutations in the iPS cell lines we generated for you. If you are looking to use disease-relevant cell types in your research, you've come to the right place. We can efficiently differentiate iPS cells to multiple important cell lineages, and provide you with cryopreserved cells or ready-to-use plated cells in 96-well format.

What we can do for you

Source specific donor samples (e.g. fibroblasts, blood cells and iPS cells)

Reprogram cells using a non-integrating, zero-footprint system

Provide more than one colony from a single donor

Sequence multiple locations of the genome of iPS cell lines

Karyotyping

Genome editing and creation of isogenic cell lines with CRISPR-Cas system Find out more

Directed differentiation of iPS cells to multiple cell types, including cardiomyocytes, endothelial cells and cerebral cortical, dopaminergic, spinal motor and sensory neural cells Find out more

The turnaround time for reprogramming is usually 2 to 3 months. At least three frozen vials of donor cells per sample will be needed. One vial will be thawed and tested for mycoplasma. We will start reprogramming once the quarantine is completed. The culture will be monitored daily for 3 weeks for potential iPS cell colonies. Live staining with TRA-1-60 antibody will be used to evaluate the reprogramming. Positive colonies will be picked for expansion using fully defined medium and substrates. The iPS cells will be passaged for 4 times to evaluate the cell line stability and characterized with ICC of standard pluripotency markers (Oct4, Nanog and Sox2).

Talk to us about your project

A few recent papers for you to get started with iPS cell culture...

  • Kevin G. Chen et al.  Human Pluripotent Stem Cell Culture: Considerations for Maintenance, Expansion, and Therapeutics.  Cell Stem Cell  14 , 13-26 (2014)
  • Jeanette Beers et al.  Passaging and colony expansion of human pluripotent stem cells by enzyme-free dissociation in chemically defined culture conditions.  Nature Protocols  7 , 2029–2040 (2012)
  • Takamichi Miyazaki et al.  Laminin E8 fragments support efficient adhesion and expansion of dissociated human pluripotent stem cells.  Nature Communications (2012)

What our customers say...

"I've been able to grow all the cells that Axol generated for me from my patient lines. All the cells provided grew well and I'm very happy with the service as well as the quality of the cells."

Dr David Millar, Human Molecular Genetics, Cardiff University

"Axol has provided us with more than a fast and reliable supply of functional cortical human iPSC derived neurons. It has also given us access to the collective expertise of Axol, whom we now view as much a collaborator as a company”

Dr Eric Hill, Clinical and Systems Neuroscience, Aston University

"Axol Bioscience has been a valued and important supplier to our lab’s research since the company was founded. We began working with sample product and were rapidly convinced by the quality of the cells, such that we now have long term orders in place for 2 neural precursor lines. Additionally, we have recommended Axol cells to several of our closest collaborators. The cells supplied to us by Axol represent exceptional value for money, but, most importantly, save us a great deal of valuable research time. Throughout our dealings with the company, service and support have always been excellent.”

Dr Paul Charlesworth, Physiology, University of Cambridge