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Stem Cells in Drug Discovery 2016

Rising to the Challenges of Human iPSC-derived Cells for Tox & Drug Screening

We presented 'in the field' data on our portfolio of human iPSC and primary cells demonstrating their proven ease of use, reliability and consistency as meaningful drug discovery tools. Here, we overcome the challenges of cell line variability and address the needs for cell scale-up in assay campaigns.

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Rising to the challenges of human iPSC-derived cells in toc and drug discovery screening

Pharmacological responses in cultured human iPSC-derived cortical neurons using multi-electrode array

The functional network of human induced pluripotent stem cell (hiPSC)-derived neurons is a potentially powerful in vitro model for evaluating disease mechanisms and drug responses. However, the culture time required for the full functional maturation of individual neurons and networks is uncertain. We investigated the development of spontaneous electrophysiological activity and pharmacological responses for over 1 year in culture using multi-electrode arrays (MEAs). The complete maturation of spontaneous firing, evoked responses, and modulation of activity by glutamatergic and GABAergic receptor antagonists/agonists required 20–30 weeks. At this stage, neural networks also demonstrated epileptiform synchronized burst firing (SBF) in response to pro-convulsants and SBF suppression using clinical anti-epilepsy drugs. Our results reveal the feasibility of long-term MEA measurements from hiPSC-derived neuronal networks in vitro for mechanistic analyses and drug screening. However, developmental changes in electrophysiological and pharmacological properties indicate the necessity for the international standardization of culture and evaluation procedures.

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Serum-Free Human iPSC-Derived Cardiomyocytes for Contactless in vitro Testing

We conducted a series of experimental procedures to examine the characteristics and potential applications of human iPSC-derived cardiomyocytes for cardiovascular research.

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