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Improving models of microglia: the development of physiologically functional human iPSC-derived microglia
Microglia are commonly described as the immune cells of the brain. Under physiological conditions, they are vigilant guard keepers of their microenvironment, seeking out invading pathogens and clearing up cell debris, apoptotic cells, and misfolded proteins by migrating, phagocytosing, and producing cytokines and neurotrophins; all to maintain a homeostatic balance in the CNS.
Axol’s method for generating hiPSC-derived microglia mimics the in vivo pathway of development for brain resident macrophages and produces high yields of microglia that are homogenous and representative of primary human microglia in vitro.
Human induced pluripotent stem cell-derived sensory neurons (hiPSC-sensory neurons) offer a physiologically relevant in vitro human model of pain perception. The dorsal root ganglion (DRG) is the collection of sensory neuron cell bodies which project axons into the peripheral nervous system. These sensory neurons express key and unique nociceptors which are implicated in chronic pain conditions.
Here we present data on the characterization of hiPSC-sensory neurons assessing the expression and function of the DRG-specific voltage-gated sodium channels (Nav1.7, Nav1.8 and Nav1.9) and transient receptor potential (TRP) ion channels, TRPV1, TRPA1 and TRPM8. Functional responses were evaluated against typical pain inducing molecules and chemotherapy drugs.
This data provides an in-depth characterization of Axol’s human iPSC-sensory neurons demonstrating a viable human cell-culture model for pioneering research and drug discovery on both nociceptive and neuropathic pain disorders.
Electrophysiological pain responses in cultured human iPSC-derived sensory neurons using high-throughput multi-electrode array system
The purpose of this study was to evaluate the physiological responses against typical pain-related molecules, temperature change and chemotherapeutic drugs Axol’s Human iPSC-derived Sensory Neurons using high-throughput multi-electrode array (MEA) system.
Dorsal root ganglion (DRG) sensory neurons are pain-related neurons and have a variety of sensory receptors that are activated by chemical, thermal, and mechanical stimuli. Establishment of pharmacological assay in pain research and drug screening is important issue. In addition, human induced pluripotent stem cell (hiPSC)-derived sensory neurons may be effectively used for drug discovery and toxicity testing.