The ability to monitor cardiomyocyte beat rate in real time
is a powerful tool for drug discovery research. To do this, human
iPSC-derived cardiomyocytes (iPSC-CMs) were cultured in a non-invasive
impedance monitoring system (xCELLigence®) to assess cardiotoxicity and
cell contractility in a 96-well plate format.
Measuring the colony forming potential of endothelial colony
forming cells (ECFCs) is an excellent method for identifying the toxic
effect of a compound on the proliferative potential of ECFCs.
Many disease pathologies are exacerbated by damage to blood
vessels whereas increased vascularization encourages cancer progression
and tumour growth, therefore there is an increased need for drugs that
can alter the proliferative population of circulating ECFCs. This
application note highlights the relevance and suitability for Human
iPSC-Derived ECFCs in the investigation for compounds that target the
anti- or pro-proliferative capabilities of ECFCs.
Three key points were covered in this study: Firstly,
whether Axol Human iPSC-derived ECFCs were able to form colonies in
vitro that hold a hierarchy of proliferative potentials equivalent to
human primary cord blood ECFCs. Secondly, the in vitro colony
forming potential of hiPSC-ECFCs was compared with human primary
umbilical cord blood (CB) ECFCs. Finally, the application of ECFCs in a
toxicity screen which identified the susceptible ECFC population.
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