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The ability to monitor cardiomyocyte beat rate in real time is a powerful tool for drug discovery research. To do this, human iPSC-derived cardiomyocytes (iPSC-CMs) were cultured in a non-invasive impedance monitoring system (xCELLigence®) to assess cardiotoxicity and cell contractility in a 96-well plate format.
Measuring the colony forming potential of endothelial colony forming cells (ECFCs) is an excellent method for identifying the toxic effect of a compound on the proliferative potential of ECFCs.
Many disease pathologies are exacerbated by damage to blood vessels whereas increased vascularization encourages cancer progression and tumour growth, therefore there is an increased need for drugs that can alter the proliferative population of circulating ECFCs. This application note highlights the relevance and suitability for Human iPSC-Derived ECFCs in the investigation for compounds that target the anti- or pro-proliferative capabilities of ECFCs.
Three key points were covered in this study: Firstly, whether Axol Human iPSC-derived ECFCs were able to form colonies in vitro that hold a hierarchy of proliferative potentials equivalent to human primary cord blood ECFCs. Secondly, the in vitro colony forming potential of hiPSC-ECFCs was compared with human primary umbilical cord blood (CB) ECFCs. Finally, the application of ECFCs in a toxicity screen which identified the susceptible ECFC population.
Human iPSC-derived cardiomyocytes: A comparison with primary cells and applications in standard and 3D culture models
The University Hospital Bern studies the mechanisms of cancer therapy-associated cardiotoxicity, and wants to study this in cardiomyocytes, thus they always endeavor to use the most relevant in vitro culture systems. Therefore, they have recently started to develop a 3D-culture model using hiPSC-derived cardiomyocytes and have tested this system in comparison with mature primary cells.
Induced pluripotent stem cell-derived endothelial colony forming cells offer a robust and physiologically relevant research tool
Human iPSC-Derived Endothelial Colony Forming Cells (ECFCs) (Axol Bioscience) are highly expandable and show comparable expression and functionality to primary cells, providing a robust and physiologically relevant tool for use in numerous applications. ECFCs are rare circulating endothelial cells that display a hierarchy of clonal proliferative potential and possess in vivo vessel-forming ability upon implantation. In numerous animal models of disease, human ECFCs have demonstrated the capacity to promote revascularization and reperfusion to injured vascular beds via direct integration and/or through paracrine effects.
Human umbilical cord blood is enriched in circulating ECFCs compared to adult peripheral blood. We have recently reported that ECFCs displaying properties similar to cord blood can be obtained from human ESCs and iPSCs. Using defined serum-free culture medium and sequential addition of specific growth factors, we’ve identified ECFC precursors within 12 days of iPSC differentiation. These iPSC-derived ECFCs display clonogenic proliferative potential and in vivo vessel forming ability similar to cord blood ECFCs and promote vascular repair and regeneration in multiple animal models of human disease.
A drug discovery platform for the identification of novel infarct sparing agents for treatment of ischemic heart disease
Any intervention aiming to protect the myocardium by reducing cardiomyocyte death following myocardial infarction (MI) would benefit cardiac repair and functional recovery. Therefore, identification of drugs that may protect the heart during an ongoing MI or reperfusion of the culprit coronary is highly relevant in the clinical setting. We have shown that the natural compound Celastrol protects the heart from permanent ischemia-induced death in vivo in a rat model of MI and in vitro in H9c2 rat cardiomyoblasts. Based on these findings, we set to validate Celastrol and expand our search for cardioprotective drugs through a series of clinically relevant tests (ischemia/reperfusion (I/R)), using high throughput screening equipment and validation in appropriate cell lines (iPSC-derived human mature cardiomyocytes).
Compounds are screened in viability assays using pre-treated H9c2 placed under hypoxic (<1% O2; 48hr) and oxidative stress (<4mM H2O2, 60min). Protective compounds are then subjected to an I/R stress assay (<1% O2, low serum/glucose 18h followed by 6h normal culture). Results are validated using iPSC-derived cardiomyocytes (Axol Bioscience). Viability is quantified using the live/dead kit with results analyzed by automated high content screening (Operetta). Next, a secondary reporter assay is used to quantitate the protective heat shock and antioxidant cellular responses (HSE and ARE reporter assays; SABiosciences). Compounds are assayed for their potency to induce protective proteins, activate survival kinases and tested in their ability to inactivate the opening of the mitochondrial permeability transition pore (mPTP assay).
Celastrol (10-6M) pre-treatment increases H9c2 viability submitted to hypoxic stress by 6.7%, oxidative stress by 14.5% and I/R stress by 18% (p<0.05); reduces troponin content by 28.8% in I/R culture media, and increases viability by 7% in iPSC-derived cardiomyocytes submitted to I/R. Celastrol (10-6M) induces a 35.8 and 3.1-fold increase in HSE and ARE reporters (p<0.001), increases protective HO1 (22.5 fold) and HSP70 (11.9 fold) protein expression, as well as pERK (2 fold) and pAKT (8.6 fold) activation. Celastrol prevents the opening of the mPTP pores in ionomycin-challenged H9c2.
We have identified and validated Celastrol as a novel infarct sparing agent and identified as well other analog compounds with superior potency. These will be tested and compared in vivo as a first step towards developing a novel drug designed as a first line medication for the treatment of MI and adjunct therapy to reperfusion procedures.
Effects of transient hypoxia/ischemia on inducible human pluripotent stem cell (iPSC)-derived cardiomyocytes
Hypoxia/ischemia is a central elicitor of ischemia/reperfusion (I/R) injury in the human heart. However, there is an apparent lack of suitable in vitro models for the investigation of I/R induced cellular mechanisms in human cardiac cells. We investigate whether inducible human pluripotent stem cell (iPSC)-derived cardiomyocytes are susceptible to physiologically defined transient hypoxic/ischemic conditions.
Spontaneously contracting iPSC-derived cardiomyocytes were subjected to enzymatically induced hypoxic/ischemic conditions by using glucose oxidase (GO, 2U/ml) and catalase (CAT, 120U/ml). Morphological assessment and measurements of LDH activity released from damaged cells (Cytotoxicity Detection Kit; Roche, Mannheim, Germany) were used for evaluating and quantifying hypoxia/ischemia induced cytotoxicity (Figure 1).
Hypoxic/ischemic conditions were rapidly established after the addition of GO/CAT and resulted in pO2 levels <10mmHg after 30 minutes (lasting for at least 4 hours), a gradual decrease of glucose concentration (from 4g/l to <1g/l after 4 hours) and a decline of pH from 7.65 to 6.98. iPSCderived cardiomyocytes showed spontaneous contractions after 10 days in culture with a beating frequency of 1520 contractions/min under normoxia as well as under hypoxic/ischemic conditions (normoxia: 18.40±3.41 bpm; hypoxia/ischemia: 15.50±2.60bpm; low resolution video file). Directly after replacing the hypoxic/ischemic medium by normoxic culture medium, frequency of contractions increased by 2fold in the hypoxia/ischemia group (normoxia: 16.72±0.93bpm; hypoxia/ischemia: 31.58±0.75bpm). 24 hours after the 4 hour hypoxia period, iPSC-derived cardiomyocytes showed clear morphological signs of cell damage such as cell rounding, swelling and detachment from the growth surface. In cultures that were subjected to 4 hours of hypoxia, LDH release as a marker of cell damage was increased 3fold while iPSC-derived cardiomyocytes that were cultured under normoxic conditions did not reveal morphological changes or increased LDH release after 24 hours (normoxia: 0.10±0.00au; hypoxia/ischemia: 0.29±0.02au).
Inducible human pluripotent stem cell-derived cardiomyocytes are susceptible to transient hypoxia/ischemia in vitro. The described culture system closely resembles hypoxic/ischemic conditions invivo and may help to elucidate cellular/molecular mechanisms of ischemia/reperfusion injury as well as cardioprotective strategies.
Using defined factors OCT3/4, KLF4, SOX2 and c-MYC, adult cells from healthy and patient donors can be reprogrammed to generate induced pluripotent stem cells (iPSCs). Subsequently, these can be differentiated into a variety of cell types including cardiomyocytes. Human iPSC-derived cardiomyocytes (iPSC-CMs) can be cultured in vitro under serum-free conditions and as such, offer a platform investigate the effect of growth factors, cytokines and drugs on the development and functionality of human cardiomyocytes in vitro. Following differentiation, spontaneously beating iPS-CM’s were evaluated in 2D and 3D culture. Expression profiling by immunohistochemistry confirms the expression of cardiomyocyte selective markers including α-actinin, myosin heavy chain, atrial and ventricular myosin light chains, troponin-T and -I, β-catenin, vimentin, L-type calcium channels, connexin-40 and -43, telethonin and ankyrin repeat domain-1 (ANKRD1). Immunohistochemistry findings were validated by Western Blot for α-actinin and cardiac troponin-T expression. Additional analyses conducted, include bi-nucleate cell counts, cell form factor measurements and a comparison of plating efficiencies across a variety of substrates. The electrical activity of the iPSC-CMs was confirmed using a multi-electrode array (MEA), and the calcium dye Fluo4. One application of these cells is drug toxicity testing. To show proof of principle that this can be undertaken in a contactless manner using only genetically encoded tools, which offers several advantages compared to low throughput contact based methods with chemical dyes, we developed a simultaneous optical control/calcium imaging approach to replace the need for electrode stimulation and dyes. We are able to control the beat frequency of iPSC- CMs across the physiological range (0.3Hz – 2Hz) and can observe the anticipated effects of compounds such as Dofetilide, a known hERG inhibitor. Here, we have identified a range of characteristics in these human iPSC-CMs that confirms their ability to function as a highly-pure population of single beating human cardiomyocytes in vitro and presented evidence of a technically simple and scalable platform for cardiotoxicity screening assays.
In vitro assays and screening platforms for exploring ventricular and atrial phenotypes in iPSC-derived cardiomyocytes
Existing cardiac safety testing regimes have successfully prevented new drugs coming to market with pro-arrhythmic risk, but they are expensive (e.g. TQT clinical studies ICH-E14) and the reliance on preclinical hERG screening (ICH-S7B) is undermined by the fact that many new chemical scaffolds have been excluded from further drug development while older drugs that inhibit hERG are not associated with arrhythmia. This has prompted the FDA to implement the Comprehensive in vitro Pro Arrhythmia initiative (CiPA) which involves 3 parts: 1) High quality in vitro cardiac ion channel assays, 2) Comprehensive in silico action potential (AP) models and 3) Predictive assays using stem cell-derived cardiomyocytes.
Our presentation will focus on recent electrophysiological ion channel profiling undertaken at Metrion using human induced pluripotent stem (iPSC)-derived cardiomyocytes from Axol Bioscience. Action potentials and ionic currents were monitored using current- and voltage-clamp manual patch electrophysiology recordings, respectively. Pharmacology was assessed using representative compounds from the CiPA validation toolbox, as well as compounds selectively targeting ventricular and atrial ion channels. Molecular and biophysical properties of these cells were assessed to explore the relative composition of functional and interdependent cardiac cell phenotypes. Phenotypic measurements of impedance (contraction) and field potential (excitability) were also undertaken using the CardioExcyte96 screening platform. Profiling of ion channel expression with a variety of pharmacological tools and techniques enabled us to ascertain the physiology of iPSC-derived cardiomyocytes and assess their potential utility in more efficacious drug screening and toxicology campaigns.
In vitro assessment of excitation-contraction coupling for predicting pro-arrhythmic risk in iPSC-derived ventricular cardiomyocytes
Human induced pluripotent stem cell-derived ventricular cardiomyocytes (hiPSC-vCMs) (Axol Bioscience) offer a physiologically relevant model for predictive toxicology screening in vitro. The CardioExcyte 96 (Nanion Technologies) is a hybrid screening instrument that simultaneously records cell contractility (impedance) and the extracellular electrical field potential (EFP) in a 96-well plate. Used in combination these tools could help predict the risk of human clinical pro-arrhythmias more accurately.
Here we present data on the optimization of hiPSC-vCMs on the CardioExcyte 96. We determined seeding parameters and identified the optimal time point for analysis. Excitation-contraction coupling was then assessed in response to three standard reference compounds from the Comprehensive in vitro Pro-arrythmia Assay (CiPA) guidelines. The three compounds tested were verapamil, a mixed ion channel blocker acting upon both L-type calcium channels (ICaV) and potassium channels (IKr); nifedipine, a selective calcium channel (ICaV) blocker; and dofetilide, a selective ion channel blocker for IKr. Both verapamil and nifedipine exhibit low pro-arrhythmic risk whereas dofetilide is classified as a high risk pro-arrhythmic compound by the Cardiac Safety Consortium. The addition of each of these compounds altered contractility and electrical excitation in the hiPSC-vCMs.
Here we have demonstrated that the CardioExcyte 96, a non-invasive, label-free, high temporal resolution tool may be used in conjunction with Axol hiPSC-vCMs to predict pro-arrythmic risk in vitro.