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Application Note

How to prepare ECFC culture medium

How to prepare Endothelial colony forming cells culture medium

Application Note

Characterization of human iPSC-derived Macrophages reveals the hallmarks of macrophage morphology and function

The use of macrophages from peripheral blood or immortalized cell lines may be reducing the reproducibility and reliability of your disease research and its successful translation into new treatments.

Application Note

ICC images of iPSC-derived striatal neurons

ICC images of iPSC-derived striatal neurons showing: DARPP32, CTIP2, CALBINDIN, GABA and MAP2 staining for ax0015 and ax0018 derived striatal neurons

Application Note

Non-invasive impedance monitoring of contractility in Axol Human iPSC-Derived Cardiomyocytes

The ability to monitor cardiomyocyte beat rate in real time is a powerful tool for drug discovery research. To do this, human iPSC-derived cardiomyocytes (iPSC-CMs) were cultured in a non-invasive impedance monitoring system (xCELLigence®) to assess cardiotoxicity and cell contractility in a 96-well plate format.

Application Note

Measuring Colony Forming Potential of Human iPSC-derived ECFCs in vitro

Measuring the colony forming potential of endothelial colony forming cells (ECFCs) is an excellent method for identifying the toxic effect of a compound on the proliferative potential of ECFCs.

Many disease pathologies are exacerbated by damage to blood vessels whereas increased vascularization encourages cancer progression and tumour growth, therefore there is an increased need for drugs that can alter the proliferative population of circulating ECFCs. This application note highlights the relevance and suitability for Human iPSC-Derived ECFCs in the investigation for compounds that target the anti- or pro-proliferative capabilities of ECFCs.

Three key points were covered in this study: Firstly, whether Axol Human iPSC-derived ECFCs were able to form colonies in vitro that hold a hierarchy of proliferative potentials equivalent to human primary cord blood ECFCs. Secondly, the in vitro colony forming potential of hiPSC-ECFCs was compared with human primary umbilical cord blood (CB) ECFCs. Finally, the application of ECFCs in a toxicity screen which identified the susceptible ECFC population.

Application Note

Transfection of Axol Cortical Neural Stem Cells (hNSCs)

Investigation into the suitability of lipid-based transfection reagents for use with Axol Human Neural Progenitor Cells (hNPCs).

Application Note

Comparative transcriptome analysis of the expression profiles of iPS cells vs Axol human neural progenitor cells

Find the results of our comparative transcriptome analysis of the expression profiles of iPS cells vs Axol human neural progenitor cells. We provide links to the primary datasets on NCBI GEO and the summary plots of our findings.

Application Note

Study of neurite dynamics using Axol Human Neural Progenitor Cells using the IncuCyte NeuroTrack Platform

The study of neurite dynamics is elemental to the investigation of neuropathological disorders, neuronal injury and regeneration, embryonic development, and neuronal differentiation. Measurements of neurite dynamics are routinely used as a screening assay in neurotherapeutic drug discovery, and changes in neurite length and branching can predict neurotoxicity and neuroprotective effects induced by a compound.

Application Note

Application of Axol Neural Cell Culture in Multi-electrode Arrays for Studying Network Electrophysiology

Synaptic connectivity and action potential propagation were examined, in collaboration with Ole Paulsen's lab at the University of Cambridge, using MEA technology.

Application Note

Demonstration of 3D Culture of Axol hNPCs and hCCNs in a collagen gel using TAP Biosystems RAFT technology

The recapitulation of the complex microenvironment in which neural cells exists allows 3D culture systems to bridge the gap between traditional cell culture approaches and in vivo models such as transgenic mice. By mimicking the "stem cell niche" and physiological environments both mechanically and spatially, the crosstalk between the cells and environment leads to a closer scenario to what can be seen in the brain.