The use of macrophages from peripheral blood or immortalized cell lines may be reducing the reproducibility and reliability of your disease research and its successful translation into new treatments.
The ability to monitor cardiomyocyte beat rate in real time
is a powerful tool for drug discovery research. To do this, human
iPSC-derived cardiomyocytes (iPSC-CMs) were cultured in a non-invasive
impedance monitoring system (xCELLigence®) to assess cardiotoxicity and
cell contractility in a 96-well plate format.
Measuring the colony forming potential of endothelial colony
forming cells (ECFCs) is an excellent method for identifying the toxic
effect of a compound on the proliferative potential of ECFCs.
Many disease pathologies are exacerbated by damage to blood
vessels whereas increased vascularization encourages cancer progression
and tumour growth, therefore there is an increased need for drugs that
can alter the proliferative population of circulating ECFCs. This
application note highlights the relevance and suitability for Human
iPSC-Derived ECFCs in the investigation for compounds that target the
anti- or pro-proliferative capabilities of ECFCs.
Three key points were covered in this study: Firstly,
whether Axol Human iPSC-derived ECFCs were able to form colonies in
vitro that hold a hierarchy of proliferative potentials equivalent to
human primary cord blood ECFCs. Secondly, the in vitro colony
forming potential of hiPSC-ECFCs was compared with human primary
umbilical cord blood (CB) ECFCs. Finally, the application of ECFCs in a
toxicity screen which identified the susceptible ECFC population.
Find the results of our comparative transcriptome analysis
of the expression profiles of iPS cells vs Axol human neural progenitor
cells. We provide links to the primary datasets on NCBI GEO and the
summary plots of our findings.
The study of neurite dynamics is elemental to the investigation of
neuropathological disorders, neuronal injury and regeneration, embryonic
development, and neuronal differentiation. Measurements of neurite
dynamics are routinely used as a screening assay in neurotherapeutic
drug discovery, and changes in neurite length and branching can predict
neurotoxicity and neuroprotective effects induced by a compound.
Synaptic connectivity and action potential propagation were examined, in
collaboration with Ole Paulsen's lab at the University of Cambridge,
using MEA technology.
The recapitulation of the complex microenvironment in which neural cells
exists allows 3D culture systems to bridge the gap between traditional
cell culture approaches and in vivo models such as transgenic mice. By
mimicking the "stem cell niche" and physiological environments both
mechanically and spatially, the crosstalk between the cells and
environment leads to a closer scenario to what can be seen in the brain.
Currently there are no definitive therapies for CNS (central
nervous system) and spinal cord injuries. The aim of a neural organoid
is to overcome limitations with current therapies and provide better
solutions for a damaged human CNS or a spinal cord injury. A neural
organoid might also be useful in conducting a neurotoxicity test for
chemical agents.
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