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Characterization of human iPSC-Derived Microglia using live-cell imaging reveals hallmarks of morphology and function
This study characterized human iPSC-derived microglia (Axol Bioscience) using IncuCyte® Live Cell assays (Essen BioScience, part of the Sartorius Group) to validate their morphological and functional features, offering insights into their suitability for immune and neurodegenerative disease research.
Axol Human iPSC-Derived Sensory Neurons were cultured on a multiple-electrode array platform (Alpha MED Scientific) in order to investigate the potential of these cells to be used as an in vitro model that can recapitulate the sensation of human pain. This study showed that cultured Axol human iPSC-Derived Sensory Neurons display the typical characteristics and firing responses of human sensory neurons.
Currently there are no definitive therapies for CNS (central nervous system) and spinal cord injuries. The aim of a neural organoid is to overcome limitations with current therapies and provide better solutions for a damaged human CNS or a spinal cord injury. A neural organoid might also be useful in conducting a neurotoxicity test for chemical agents.
Demonstration of 3D Culture of Axol hNPCs and hCCNs in a collagen gel using TAP Biosystems RAFT technology
The recapitulation of the complex microenvironment in which neural cells exists allows 3D culture systems to bridge the gap between traditional cell culture approaches and in vivo models such as transgenic mice. By mimicking the "stem cell niche" and physiological environments both mechanically and spatially, the crosstalk between the cells and environment leads to a closer scenario to what can be seen in the brain.
Application of Axol Neural Cell Culture in Multi-electrode Arrays for Studying Network Electrophysiology
Synaptic connectivity and action potential propagation were examined, in collaboration with Ole Paulsen's lab at the University of Cambridge, using MEA technology.
Study of neurite dynamics using Axol Human Neural Progenitor Cells using the IncuCyte NeuroTrack Platform
The study of neurite dynamics is elemental to the investigation of neuropathological disorders, neuronal injury and regeneration, embryonic development, and neuronal differentiation. Measurements of neurite dynamics are routinely used as a screening assay in neurotherapeutic drug discovery, and changes in neurite length and branching can predict neurotoxicity and neuroprotective effects induced by a compound.
Comparative transcriptome analysis of the expression profiles of iPS cells vs Axol human neural progenitor cells
Find the results of our comparative transcriptome analysis of the expression profiles of iPS cells vs Axol human neural progenitor cells. We provide links to the primary datasets on NCBI GEO and the summary plots of our findings.
Investigation into the suitability of lipid-based transfection reagents for use with Axol Human Neural Progenitor Cells (hNPCs).
Measuring the colony forming potential of endothelial colony forming cells (ECFCs) is an excellent method for identifying the toxic effect of a compound on the proliferative potential of ECFCs.
Many disease pathologies are exacerbated by damage to blood vessels whereas increased vascularization encourages cancer progression and tumour growth, therefore there is an increased need for drugs that can alter the proliferative population of circulating ECFCs. This application note highlights the relevance and suitability for Human iPSC-Derived ECFCs in the investigation for compounds that target the anti- or pro-proliferative capabilities of ECFCs.
Three key points were covered in this study: Firstly, whether Axol Human iPSC-derived ECFCs were able to form colonies in vitro that hold a hierarchy of proliferative potentials equivalent to human primary cord blood ECFCs. Secondly, the in vitro colony forming potential of hiPSC-ECFCs was compared with human primary umbilical cord blood (CB) ECFCs. Finally, the application of ECFCs in a toxicity screen which identified the susceptible ECFC population.
The ability to monitor cardiomyocyte beat rate in real time is a powerful tool for drug discovery research. To do this, human iPSC-derived cardiomyocytes (iPSC-CMs) were cultured in a non-invasive impedance monitoring system (xCELLigence®) to assess cardiotoxicity and cell contractility in a 96-well plate format.