Axol's Antifade Mounting Medium with DAPI prevents rapid photobleaching of fluorescent proteins and dyes. It is compatible with a wide array of fluorochromes, enzymatic substrates, and fluorescent proteins, including many of the fluorophores used in super resolution methods like STORM, STED, and 3D-SIMM imaging. When bound to DNA, DAPI (4′,6-diamidino-2-phenylindole) produces a blue fluorescence with excitation at 360 nm and emission at 460 nm. This enables visualisation of nuclei in cells.
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