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Description
Axol Assay-Ready Expanded (ARE) Hepatocytes are primary human hepatocytes that have been expanded in vitro so large batch sizes (up to 2000 vials) are available, ensuring a reliable and consistent source of primary hepatocytes. They are ready-to-use and should be used directly for downstream applications. ARE Hepatocytes express CYP enzymes, are metabolically functional, are polarized and can be infected by the Hepatitis C virus. The CYP2D6 Overexpressing ARE Hepatocytes are available with a matched isogenic line with basal levels of CYP2D6, permitting metabolism studies that focus on the role of CYP2D6.
Product Specification
Donor gender
Female
Donor age at sampling
9 yrs
Genetic modification
Previous activation of the oncostatin M pathway
Size
5 million cells
Growth properties
Adherent
Shipping conditions
Dry ice
Storage conditions
Liquid nitrogen
Xeno free
No
Usage notes
Please see our protocol for detailed instructions. Our Thawing Medium and Maintenance Medium should be used to maintain the cells in their differentiated, fully-functional state. Culture vessels must be coated with Type I Collagen (not provided).
Condition of sale
The end user is not permitted to expand or re-freeze the cells
Axol Assay-Ready Expanded (ARE) Hepatocytes are primary human hepatocytes that have been expanded in vitro so large batch sizes (up to 2000 vials) are available, ensuring a reliable and consistent source of primary hepatocytes. They are ready-to-use and should be used directly for downstream applications. ARE Hepatocytes express CYP enzymes, are metabolically functional, are polarized and can be infected by the Hepatitis C virus. The CYP2D6 Overexpressing ARE Hepatocytes are available with a matched isogenic line with basal levels of CYP2D6, permitting metabolism studies that focus on the role of CYP2D6.
Donor gender
Female
Donor age at sampling
9 yrs
Genetic modification
Previous activation of the oncostatin M pathway
Size
5 million cells
Growth properties
Adherent
Shipping conditions
Dry ice
Storage conditions
Liquid nitrogen
Xeno free
No
Usage notes
Please see our protocol for detailed instructions. Our Thawing Medium and Maintenance Medium should be used to maintain the cells in their differentiated, fully-functional state. Culture vessels must be coated with Type I Collagen (not provided).
Condition of sale
The end user is not permitted to expand or re-freeze the cells
Read nowLevy G, Bomze D, Heinz S. Long-term culture and expansion of primary human hepatocytes. Nature Biotechnology (2015)
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