Axol's Transfection Reagent is non-toxic and as a result cell survival rate is >90%. The reagent can be directly added into complete cell medium and assayed for transfection efficiency after 24-72 hours without the need to change the media. This also makes the reagent ideally suited to high-throughput transfection.
Compared with cationic lipids, Axol's Transfection Reagent is very stable, easy to handle and resistant to serum in cell cultures, making gene transfection easy, the procedure can be completed in less than 30 minutes, and reproducible. The product is useful for both primary cells and established cell lines and can be used for
stable and transient transfection.
Reagent contains 1 x 1.5mL Transfection Reagent (1 µg/µL) and 1 x GFP-Plasmid Transfection Control (1 µg/µL). This size is
suitable for between 375 - 750 transfections.