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Description
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the V337M mutation (GTG>ATG) in the MAPT gene. This line is heterozygous for the V337M mutation where 1 allele has the mutation and the other allele is wild type. Click on the product images to see the data and further details.
The V337M mutation in MAPT has been implicated in frontotemporal dementia and may have a role in Alzheimer's disease. The mutation leads to accelerated aggregation of MAPT/tau protein into filament structures (Nacharaju et al., 1999) and makes the MAPT/tau protein more susceptible to hyperphosphorylation (Alonso et al., 2004).
Product Specification
Starting material
Dermal fibroblast
Donor gender
Female
Donor age at sampling
64 yrs
Karyotype
Normal
Reprogramming method
Episomal vector
Induction method
Monolayer & chemically defined medium
Genetic modification
Heterozygous for the MAPT V337M mutation (GTG>ATG)
Genetic modification
Contains a puromycin resistance cassette (intronic)
Size
≥1.5 million cells
Kit components
1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Growth properties
Adherent
Shipping conditions
Dry ice
Storage conditions
Liquid nitrogen
Frequently Asked Questions
For cortical neurogenesis, when do I switch from differentiation media (Diff-XF) to maintenance media NMM-XF?
Nnly move into maintenance media (NMM-XF) when at least 80% of your culture has differentiated to the cortical neurons.
I want to carry out imaging/confocal microscopy, how should I plate the cells?
Plate the cells on Readyset + Surebond (ax0052)
I want to passage and expand the iPSC-derived neural stem cells, what coating shall I use?
Always plate onto Surebond (ax0041) if you intend to detach the cells for continued expansion
I am not sure what coating I should use for iPSC-derived neural stem cells?
Surebond-XF - Xeno-free coating reagent needed for endpoint assays on plastic, this is the easiest method of coating, with no wash steps.
Surebond + Readyset - Coating reagent needed for endpoint assays on glass
Surebond- Coating reagent needed when passaging of NSCs is required
Nacharaju P, Lewis J, Easson C, Yen S, Hackett J, Hutton M, Yen SH. Accelerated filament formation from tau protein with specific FTDP-17 missense mutations. FEBS Lett. (1999)
Alonso Ad, Mederlyova A, Novak M, Grundke-Iqbal I, Iqbal K. Promotion of hyperphosphorylation by frontotemporal dementia tau mutations. J Biol Chem. (2004)
Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the V337M mutation (GTG>ATG) in the MAPT gene. This line is heterozygous for the V337M mutation where 1 allele has the mutation and the other allele is wild type. Click on the product images to see the data and further details.
The V337M mutation in MAPT has been implicated in frontotemporal dementia and may have a role in Alzheimer's disease. The mutation leads to accelerated aggregation of MAPT/tau protein into filament structures (Nacharaju et al., 1999) and makes the MAPT/tau protein more susceptible to hyperphosphorylation (Alonso et al., 2004).
Read nowNacharaju P, Lewis J, Easson C, Yen S, Hackett J, Hutton M, Yen SH. Accelerated filament formation from tau protein with specific FTDP-17 missense mutations. FEBS Lett. (1999)
Read nowAlonso Ad, Mederlyova A, Novak M, Grundke-Iqbal I, Iqbal K. Promotion of hyperphosphorylation by frontotemporal dementia tau mutations. J Biol Chem. (2004)
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