axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM

axolGEM iPSC-Derived Neural Stem Cells MAPT V337M HOM (1.5 million cells) and Neural Plating-XF Medium (30 mL).

This line is available to pre-order. Contact us for further details.

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Description

Human iPSC-Derived Neural Stem Cells that have been genetically edited using CRISPR-Cas9 technology to introduce the V337M mutation (GTG>ATG) in the MAPT gene. This line is homozygous for the V337M mutation so both alleles contain the mutation. Click on the product images to see the data and further details.

The V337M mutation in MAPT has been implicated in frontotemporal dementia and may have a role in Alzheimer's disease. The mutation leads to accelerated aggregation of MAPT/tau protein into filament structures (Nacharaju et al., 1999) and makes the MAPT/tau protein more susceptible to hyperphosphorylation (Alonso et al., 2004).

Click here to see more data on Axol Neural Stem Cells

Product Specification

Starting material Dermal fibroblast
Donor gender Female
Donor age at sampling 64 yrs
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification Homozygous for the MAPT V337M mutation (GTG>ATG)
Genetic modification Contains a puromycin resistance cassette (intronic)
Size ≥1.5 million cells
Kit components 1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen

Technical Resources

References

  • Read nowAlonso Ad, Mederlyova A, Novak M, Grundke-Iqbal I, Iqbal K. Promotion of hyperphosphorylation by frontotemporal dementia tau mutations. J Biol Chem. (2004)
  • Read nowNacharaju P, Lewis J, Easson C, Yen S, Hackett J, Hutton M, Yen SH. Accelerated filament formation from tau protein with specific FTDP-17 missense mutations. FEBS Lett. (1999)