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Description
Axol Cardiomyocyte Maintenance Medium has been developed for optimal culturing and maintenance of our Human iPSC-Derived Cardiomyocytes. Cardiomyocyte Maintenance Medium does not contain serum or antibiotics.
Product Specification
Size
500 mL basal medium + 10 mL supplement
Phenol red indicator
Yes
Xeno free
No
Sterility
Sterile
Usage notes
Store the basal medium at 4ºC and the supplement at -80ºC
Frequently Asked Questions
Issues with cardiomyocyte aggregates after thawing
1. At the resuspension step, using P1000 and 1ml of plating medium to pipette up and down at least 5 times gently against the falcon tube which would partially disintegrate the aggregates;
2. In the counting step do not count the large aggregates and seed the culture vessel at a density of 50,000 cells/cm2 as instructed by the protocol;
3. The day after plating (Day 1), change Plating Medium to Cardiomocyte Maintenance Medium which would remove debris and cells that didn't attach (including large aggregates).
4. Using the recommended seeding density we have achieved over 70% confluence culture after plating.
Can I freeze and thaw cardiomyocytes after culturing?
No, because the extra freeze/thaw cycle would effect the viability of the cells.
Chang Y, Broyles CN, Brook FA et al., Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics. PLoS One (2017)
Axol Cardiomyocyte Maintenance Medium has been developed for optimal culturing and maintenance of our Human iPSC-Derived Cardiomyocytes. Cardiomyocyte Maintenance Medium does not contain serum or antibiotics.
Read now
Chang Y, Broyles CN, Brook FA et al., Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics. PLoS One (2017)
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