The CD3 epitope is expressed on the epsilon chain of the CD3/T cell antigen receptor (TcR) complex. CD3 is present on 65-85% of thymocytes and has a mitogenic effect on peripheral blood T cells. CD3 indentifies human T cells expressing the 22-28,000 M.W. surface antigen. CD4 identifies human helper/inducer T cells expressing the 60,000 M. W. surface antigen (HLA class II reactive). CD4 is present in low density on monocytes. CD45 identifies all human leukocytes, lymphocytes, monocytes, polymorphonuclear cells, eosinphils and basophils in peripheral blood expressing the 180-220K M.W. T200 family surface antigen. CD45 is also present on thymus,spleen, tonsil and progenitor cells in bone marrow.
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