The ATP-dependent oxidation of luciferin by luciferase produces light, and this system is employed as a reporter in plants, bacteria, and mammalian cells. This substrate for the Firefly luciferase enzyme utilizes ATP and Mg2+ as co-factors and emits a characteristic yellow-green emission in the presence of oxygen, which shifts to red light in vivo at 37°C. Because chemiluminescent techniques are virtually background-free, this reporter gene system is ideal for detecting low-level gene expression. CAS Number: 2591-17-5; Synonyms: Firefly luciferin; D-Luciferin; 2591-17-5; Photinus luciferin; Luciferin; D-Luciferin free acid Molecular Formula: C11H8N2O3S2; Molecular Weight: 280.316 g/mol InChI Key: IWJYWBVPCGUPLO-BFUDMSGGSA-N
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