Axol Dental Pulp Stem Cell Culture Medium permits maximal recovery, viability and proliferation of human dental pulp stem cells. The medium permits Axol Dental Pulp Stem Cells to be passaged a further 3 times and still maintain their differentiation potential to multiple cell lineages. The medium does not contain phenol red or antimicrobial agents since these can negatively affect cell health. For convenience, the medium is fully supplemented and ready to use. It can be aliquoted and stored at -20°C for up to 6 months.