• Search

DNA Topoisomerase 1

DNA Topoisomerase 1 releases the supercoiling and torsional tension of DNA introduced during DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. It introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells.

Product Code: ax1082049 (100 µg) Categories: .

Payment Options

We accept Visa and MasterCard in our online store.

Worldwide Shipping

We can get your purchases to you wherever you are.

Customer Care

As an Axol customer you have access to our support team and product specialists.

Product Specification

Application Immunohistochemistry (paraffin-embedded)
Host Rabbit
Size 100 µg
Species reactivity Human
Isotype Ig
Shipping conditions Ambient temperature
Storage conditions -20°C