Axol Fibronectin Coating Solution can be used to promote attachment of a wide variety of cells types. It has been validated to promote attachment of Axol iPSC-Derived Ventricular Cardiomyocytes after thawing.
The human fibronectin coating solution is cell culture tested and is negative for human viruses and microbial contamination.
The recommended working concentration varies depending on cell type:
ax2015 Human iPSC-Derived Endothelial Colony Forming Cells 1:100
ax2019 Human iPSC-Derived Endothelial Colony Forming Cells 1:100
ax2505 Human iPSC-Derived Ventricular Cardiomyocytes 1:100 (can be further optimized for individual experiments)
ax9001 Mesenchymal Stem Cells (MSCs) (pre-adipocyte) 1:50
ax9002 MSCs (adipose tissue-derived) 1:50
ax9003 MSCs (umbilical cord-derived) 1:50
ax9004 MSCs (bone marrow-derived) 1:50
Dilute Fibronectin Coating Solution in sterile water and mix well.
Completely cover the wells to coat and incubate overnight at 37oC in a humidified incubator. Aspirate the coating solution prior to seeding the cells.
Once diluted, use immediately as diluted Fibronectin Coating Solution is not stable for extended periods of time.
Fibronectin Coating Solution should be stored in small concentrated aliquots to reduce freeze-thaw cycles.