We accept Visa and MasterCard in our online store.
Worldwide Shipping
We can get your purchases to you wherever you are.
Customer Care
As an Axol customer you have access to our support team and product specialists.
Description
MCF7/182R-6 is a fulvestrant-resistant MCF7 breast cancer cell line. The parental MCF7/S0.5 line was treated long-term with 100 nM fulvestrant and the surviving clone was expanded as a resistant cell line. To maintain a high level of resistance, the cells are cultured in the presence of 100 nM fulvestrant.
Condition of sale: the end user is not permitted to transfer or re-freeze the cells.
Product Specification
Donor gender
Female
Donor age at sampling
69 yrs
Genetic modification
None
Size
1 million cells
Growth properties
Adherent
Disease information
Malignant adenocarcinoma derived from pleural effusion
General notes
Expresses the estrogen receptor (ER); resistant to fulvestrant at 100 nM
Shipping conditions
Dry ice
Storage conditions
Liquid nitrogen
Usage notes
Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin. Addition of 100 nM fulvestrant is required to maintain a high level of resistance
Condition of sale
The end user is not permitted to transfer or re-freeze the cells
Lykkesfeldt AE, Larsen SS, Briand P. Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment. International Journal of Cancer (1995)
Nabha SM, Glaros S, Hong M et al. Upregulation of PKC-delta contributes to antiestrogen resistance in mammary tumor cells. Oncogene (2005)
Frogne T, Jepsen JS, Larsen SS. Antiestrogen-resistant human breast cancer cells require activated protein kinase B/Akt for growth. Endocrine-related Cancer (2005)
Frankel LB, Lykkesfeldt AE, Hansen JB. Protein Kinase C alpha is a marker for antiestrogen resistance and is involved in the growth of tamoxifen resistant human breast cancer cells. Breast Cancer Research and Treatment (2007)
Frogne T, Benjaminsen RV, Sonne-Hansen K. Activation of ErbB3, EGFR and Erk is essential for growth of human breast cancer cell lines with acquired resistance to fulvestrant. Breast Cancer Research and Treatment (2009)
Sonne-Hansen K, Norrie IC, Emdal KB. Breast cancer cells can switch between estrogen receptor alpha and ErbB signaling and combined treatment against both signaling pathways postpones development of resistance. Breast Cancer Research and Treatment (2010)
Thrane S, Pedersen AM, Thomsen MB et al. A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells. Oncogene (2015)
MCF7/182R-6 is a fulvestrant-resistant MCF7 breast cancer cell line. The parental MCF7/S0.5 line was treated long-term with 100 nM fulvestrant and the surviving clone was expanded as a resistant cell line. To maintain a high level of resistance, the cells are cultured in the presence of 100 nM fulvestrant.
Condition of sale: the end user is not permitted to transfer or re-freeze the cells.
Donor gender
Female
Donor age at sampling
69 yrs
Genetic modification
None
Size
1 million cells
Growth properties
Adherent
Disease information
Malignant adenocarcinoma derived from pleural effusion
General notes
Expresses the estrogen receptor (ER); resistant to fulvestrant at 100 nM
Shipping conditions
Dry ice
Storage conditions
Liquid nitrogen
Usage notes
Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin. Addition of 100 nM fulvestrant is required to maintain a high level of resistance
Condition of sale
The end user is not permitted to transfer or re-freeze the cells
Read nowLykkesfeldt AE, Larsen SS, Briand P. Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment. International Journal of Cancer (1995)
Read nowNabha SM, Glaros S, Hong M et al. Upregulation of PKC-delta contributes to antiestrogen resistance in mammary tumor cells. Oncogene (2005)
Read nowFrogne T, Jepsen JS, Larsen SS. Antiestrogen-resistant human breast cancer cells require activated protein kinase B/Akt for growth. Endocrine-related Cancer (2005)
Read nowFrankel LB, Lykkesfeldt AE, Hansen JB. Protein Kinase C alpha is a marker for antiestrogen resistance and is involved in the growth of tamoxifen resistant human breast cancer cells. Breast Cancer Research and Treatment (2007)
Read nowFrogne T, Benjaminsen RV, Sonne-Hansen K. Activation of ErbB3, EGFR and Erk is essential for growth of human breast cancer cell lines with acquired resistance to fulvestrant. Breast Cancer Research and Treatment (2009)
Read nowSonne-Hansen K, Norrie IC, Emdal KB. Breast cancer cells can switch between estrogen receptor alpha and ErbB signaling and combined treatment against both signaling pathways postpones development of resistance. Breast Cancer Research and Treatment (2010)
Read nowThrane S, Pedersen AM, Thomsen MB et al. A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells. Oncogene (2015)
How to Order
To place an order or request a quotation simply add the desired products to your shopping cart and then click "Process Checkout" to provide the billing and shipping information. On the payment page you can then either pay immediately or download a quotation.
Order Confirmed
Thank you for your order. We have received your purchase order successfully and will start processing it as soon as possible.
We will be in touch shortly.
Issue with Order Detected
There was an issue with your submitted order as follows: Unidentified Error Please resolve this issue and try again. If you are unable to do so, please contact us via the contact form and we'll do our best to resolve your issue.
