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Description
MCF7/182R-6 is a fulvestrant-resistant MCF7 breast cancer cell line. The parental MCF7/S0.5 line was treated long-term with 100 nM fulvestrant and the surviving clone was expanded as a resistant cell line. To maintain a high level of resistance, the cells are cultured in the presence of 100 nM fulvestrant.
Condition of sale: the end user is not permitted to transfer or re-freeze the cells.
Product Specification
Donor gender
Female
Donor age at sampling
69 yrs
Genetic modification
None
Size
1 million cells
Growth properties
Adherent
Disease information
Malignant adenocarcinoma derived from pleural effusion
General notes
Expresses the estrogen receptor (ER); resistant to fulvestrant at 100 nM
Shipping conditions
Dry ice
Storage conditions
vapour phase nitrogen
Usage notes
Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin. Addition of 100 nM fulvestrant is required to maintain a high level of resistance
Condition of sale
The end user is not permitted to transfer or re-freeze the cells
Lykkesfeldt AE, Larsen SS, Briand P. Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment. International Journal of Cancer (1995)
Nabha SM, Glaros S, Hong M et al. Upregulation of PKC-delta contributes to antiestrogen resistance in mammary tumor cells. Oncogene (2005)
Frogne T, Jepsen JS, Larsen SS. Antiestrogen-resistant human breast cancer cells require activated protein kinase B/Akt for growth. Endocrine-related Cancer (2005)
Frankel LB, Lykkesfeldt AE, Hansen JB. Protein Kinase C alpha is a marker for antiestrogen resistance and is involved in the growth of tamoxifen resistant human breast cancer cells. Breast Cancer Research and Treatment (2007)
Frogne T, Benjaminsen RV, Sonne-Hansen K. Activation of ErbB3, EGFR and Erk is essential for growth of human breast cancer cell lines with acquired resistance to fulvestrant. Breast Cancer Research and Treatment (2009)
Sonne-Hansen K, Norrie IC, Emdal KB. Breast cancer cells can switch between estrogen receptor alpha and ErbB signaling and combined treatment against both signaling pathways postpones development of resistance. Breast Cancer Research and Treatment (2010)
Thrane S, Pedersen AM, Thomsen MB et al. A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells. Oncogene (2015)
MCF7/182R-6 is a fulvestrant-resistant MCF7 breast cancer cell line. The parental MCF7/S0.5 line was treated long-term with 100 nM fulvestrant and the surviving clone was expanded as a resistant cell line. To maintain a high level of resistance, the cells are cultured in the presence of 100 nM fulvestrant.
Condition of sale: the end user is not permitted to transfer or re-freeze the cells.
Donor gender
Female
Donor age at sampling
69 yrs
Genetic modification
None
Size
1 million cells
Growth properties
Adherent
Disease information
Malignant adenocarcinoma derived from pleural effusion
General notes
Expresses the estrogen receptor (ER); resistant to fulvestrant at 100 nM
Shipping conditions
Dry ice
Storage conditions
vapour phase nitrogen
Usage notes
Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin. Addition of 100 nM fulvestrant is required to maintain a high level of resistance
Condition of sale
The end user is not permitted to transfer or re-freeze the cells
Read now
Lykkesfeldt AE, Larsen SS, Briand P. Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment. International Journal of Cancer (1995)
Read now
Nabha SM, Glaros S, Hong M et al. Upregulation of PKC-delta contributes to antiestrogen resistance in mammary tumor cells. Oncogene (2005)
Read now
Frogne T, Jepsen JS, Larsen SS. Antiestrogen-resistant human breast cancer cells require activated protein kinase B/Akt for growth. Endocrine-related Cancer (2005)
Read now
Frankel LB, Lykkesfeldt AE, Hansen JB. Protein Kinase C alpha is a marker for antiestrogen resistance and is involved in the growth of tamoxifen resistant human breast cancer cells. Breast Cancer Research and Treatment (2007)
Read now
Frogne T, Benjaminsen RV, Sonne-Hansen K. Activation of ErbB3, EGFR and Erk is essential for growth of human breast cancer cell lines with acquired resistance to fulvestrant. Breast Cancer Research and Treatment (2009)
Read now
Sonne-Hansen K, Norrie IC, Emdal KB. Breast cancer cells can switch between estrogen receptor alpha and ErbB signaling and combined treatment against both signaling pathways postpones development of resistance. Breast Cancer Research and Treatment (2010)
Read now
Thrane S, Pedersen AM, Thomsen MB et al. A kinase inhibitor screen identifies Mcl-1 and Aurora kinase A as novel treatment targets in antiestrogen-resistant breast cancer cells. Oncogene (2015)
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