Astrocyte dysfunction has been implicated in a number of neurological conditions such as Alzheimer’s and Parkinson’s diseases, autism, amyotrophic lateral sclerosis (ALS), Rett syndrome and schizophrenia.
Axol’s Human iPSC-derived astrocytes offer a convenient and physiologically-relevant tool to study these cells as an isolated population or within co-cultures of other neural cells for analysis of complex central nervous system (CNS) circuitry.
Axol's Human iPSC-derived astrocytes express glial markers such as glial fibrillary acidic protein (GFAP), S100b, AQP4, ALDHL1L and EAAT1.
They have also been further validated for expression of other relevant markers including Vimentin, Kir4.1 (potassium channel) and CD44.
These cells exhibit low expression of Nestin, a marker of neural stem cells, and are tested to have very low or negative expression for MAP2, a marker of neurons.
The expression of these markers highlights that
Axol's Human iPSC astrocytes are physiologically representative of human primary astrocytes.
Axol's astrocytic population is comprised of a mixture of grey and white matter astrocytes with a preference towards WHITE MATTER, focusing on those cellular populations that modulate the distribution of action potentials coordinating communication between different brain regions, acting as a relay for input/output signals
MEA Characterization of Cerebral Cortical Neuron-Astrocyte Co-culture