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Human iPSC-Derived Atrial Cardiomyocytes (Male)

All Axol's cardiomyocytes are derived from human iPSCs and ONLY human iPSCs. We do NOT use animal cells or embryonic stem cells (ESCs).

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Human iPSC-derived Atrial Cardiomyocytes (1 million cells per vial)


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Description

Axol iPSC-Derived Atrial Cardiomyocytes express atrial specific markers atrial myosin light chain 2 (MLC2a) and atrial natriuretic peptide (ANP). Atrial cardiomyocytes are key for the study of atrial fibrillation such as irregular heart rate making them excellent for disease modelling. 

Our iPSC-derived Atrial Cardiomyocytes have been derived from the same newborn male donor as our highly-validated iPSC-Derived Ventricular Cardiomyocytes. Axol iPSC-derived cardiomyocytes and fully-defined, serum-free Cardiomyocyte Maintenance Medium offers researchers a complete portfolio of complementary cell types and culture reagents for disease modelling and cardiotoxicity testing.

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A29 A68, B38(Bw4) B44(Bw4), Cw8 Cw12
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size 1 million cells
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

Frequently Asked Questions

1. At the resuspension step, using P1000 and 1ml of plating medium to pipette up and down at least 5 times gently against the falcon tube which would partially disintegrate the aggregates; 2. In the counting step do not count the large aggregates and seed the culture vessel at a density of 50,000 cells/cm2 as instructed by the protocol; 3. The day after plating (Day 1), change Plating Medium to Cardiomocyte Maintenance Medium which would remove debris and cells that didn't attach (including large aggregates). 4. Using the recommended seeding density we have achieved over 70% confluence culture after plating.

No, because the extra freeze/thaw cycle would effect the viability of the cells.

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