Human iPSC-Derived Cortical Neuron Differentiation Kit

800.00

Human iPSC-Derived Cortical Neuron Differentiation Kit consisting of: Human iPSC-Derived Neural Stem Cells (Female; ax0016; 1.5 million cells), 1 bottle of Neural Plating-XF Medium (30 mL) and 1 bottle of Neural Differentiation-XF Medium (125 mL). 

Product Code: ax0026F Categories: , , .

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Description

This is a differentiation kit for generating cerebral cortical neurons

iPSC-derived under fully defined neural induction conditions

This kit from Axol enables you to derive cerebral cortical neurons from iPSC-derived neural stem / progenitor cells (NSCs) under fully defined neural induction conditions. The NSCs provided in this kit are cryopreserved in our proprietary freezing medium. After thawing and plating, the cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of 30 ~ 40 days.

Cerebral cortical neurons are implicated in numerous neurological diseases including Alzheimer’s disease, autism, epilepsy and stroke. Despite strong interest in these diseases, high quality human in vitro cell models for cortical neurophysiology have been unavailable until now. With Axol neurons and NSCs, we provide neuroscience researchers with a high quality in vitro human system to complement their existing studies of cortical development, function and diseases using animal models. 

HLA Class I type: A3 A33, B7(Bw6) B7(Bw6), Cw7 Cw7

Advantages of Cortical Neuron Differentiation Kit
 

  • Human cells

  • Available in industrial quantity

  • Ready-made

  • Supplied with extensive functional and molecular characterization data

  • Enable derivation of a spectrum of cortical neurons

  • Remain viable in culture for months to facilitate reproducible and long term studies

  • Cells from multiple donors are available for purchase allowing data comparison among biological replicates

Specifications
 

  • Catalog number: ax0026F

  • Gender: Female

  • Donor Age: New Born

  • Starting Material: Cord Blood CD34+ Cells

  • HLA Class I type:

    • A3 A33, B7(Bw6) B7(Bw6), Cw7 Cw7

  • Reprogramming Method: Episomal Plasmid

  • Neural Induction Method: Monolayer & Chemically Defined Medium

  • Genetic Modification: No

  • Size: > 2 million cryopreserved NPCs and young neurons per kit. Kit includes Plating-XF medium (ax0033) and Neural Differentiation-XF Medium (ax0034-125).

  • Presentation: Cells are provided cryopreserved in 1 mL of freezing medium

  • Storage Condition: Liquid nitrogen

  • Growth Properties: Adherent 

Applications
 

  • Studying Corticogenesis in vitro

  • Studying neural network formation and signal transmission

  • Disease modelling

  • Neurotoxicity testing

  • Preclinical drug efficacy testing

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1 Day After Thawing

14 Days After Thawing

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Product Specification

Starting material Cord blood CD34+ cells
Donor gender Female
Donor age at sampling Newborn
HLA serotype A3 A33, B7(Bw6) B7(Bw6), Cw7 Cw7
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size ≥1.5 million cells
Kit components 1 vial of ≥1.5 million Neural Stem Cells, 1x 125 mL of Neural Differentiation-XF Medium and 1 x 30 mL bottle of Plating-XF Medium
Cell type iPSC-derived neural stem cells
Growth properties Adherent

Technical Resources

References

  • Read nowOdawara A, Katoh H, Matsuda N, et al., Physiological maturation and drug responses of human induced pluripotent stem cell-derived cortical neuronal networks in long-term culture. Scientific Reports (2016)
  • Read nowOdawara A, Katoh H, Matsuda N, et al., Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons. Biochemical and Biophysical Research Communications (2015)