Human iPSC-Derived Dopaminergic Neuron Progenitor Kit

1920.00

Human iPSC-Derived Dopaminergic Neuron Progenitor Kit consisting of Dopaminergic Neuron Progenitors (1 million cells) and Dopaminergic Neuron Basal Medium (100 mL) and Supplements for optimal maturation and maintenance of dopaminergic neurons.

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Description

Axol Human iPSC-Derived Dopaminergic Neuron Progenitor Kit consists of cryopreserved, pre-differentiated Dopaminergic Neuron Progenitors together with optimized cell culture medium that promotes maturation and maintenance of dopaminergic neurons. This kit permits the generation of mature dopaminergic neurons within 12 days after thawing. The cell population generated will consist of: >80% TUJ1+ neurons, >30% tyrosine hydroxylase (TH)+ dopaminergic neurons and <5% GFAP+ astrocytes. The dopaminergic neurons can be maintained for up to 40 days in our Dopaminergic Neuron Maintenance Medium. It is recommended that the Dopaminergic Neuron Progenitors are matured for up to 5 weeks in order to obtain electrically active dopaminergic neurons.

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A2 A3 B35(Bw6) B44(Bw4) Cw4 Cw5
Karyotype Normal
Reprogramming method Episomal vector
Induction method Fully defined medium
Genetic modification None
Size 1 million cells
Kit components Human iPSC-Derived Dopaminergic Neuron Progenitors (≥1 million cells); Dopaminergic Neuron Basal Medium (100 mL) and two Supplements
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen
Usage notes Please see the protocol for detailed instructions. Axol Dopaminergic Neuron Progenitors can be cultured on various culture vessel formats including 96 well plates and either glass or plastic surfaces.

Technical Resources

References

  • Read nowSwistowski A, Peng J, Liu Q et al., Efficient Generation of Functional Dopaminergic Neurons from Human Induced Pluripotent Stem Cells Under Defined Conditions. Stem Cells (2010)