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Human iPSC-Derived Mature Astrocyte Kit

Product Code: ax0084 (1 kit) Categories: , .

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Description

Axol iPSC-Derived Mature Astrocyte Kit contains cryopreserved mature astrocytes together with optimized Astrocyte Maintenance Medium to maintain the astrocytes. The astrocytes are derived from an iPSC line from a healthy donor. More than 80% of the cells are GFAP+ (Glial Fibrillary Acidic Protein) and ≤15% of the cells are TUJ1+ (neuronal class III ß-tubulin) neurons. 

Comparing marker expression in iPSC-derived and primary astrocytes using immunocytochemistry

Expression of GFAP, S100, nestin, MAP2, SOX2, ALDH1L1, glutamine synthetase, EAAT1 and integrin beta 5 in our iPSC-derived mature astrocytes, competitor cells and primary astrocytes. (Data provided by Susanne Zach, Boehringer Ingelheim.)

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A2 A3 B35(Bw6) B44(Bw4) Cw4 Cw5
Karyotype Normal
Reprogramming method Episomal vector
Induction method Fully defined medium
Genetic modification None
Size 1 million cells
Kit components 1 vial of iPSC-Derived Mature Astrocytes (≥1 million cells), 1 x 50 mL bottle of Astrocyte Maintenance Medium (including Supplement A, Supplement B and Supplement C)
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen

Frequently Asked Questions

Mature Astrocytes have a purity based on our listed QC criteria. Our ICC standards ensure cell markers such as GFAP meet or exceed 85% based off current templates and we have a minimum of 80% viability guaranteed upon recovery in addition to sterility (mycoplasma) testing. With regards to proliferative capacity, there are many variables that can impact growth like seeding densities. When cells are in a logarithmic growth rate the population will double every 24hrs and daily monitoring is required to evaluate the estimated state of confluence.

Please follow the link to download the PDF file with APOE genotype information: https://www.axolbio.com/web/binary/saveas?model=ir.attachment&field=datas&filename_field=name&id=92400&t=1556550049465

Mature astrocyte at this stage almost stop proliferating so even you passage them, you may not get more cells but lose cells during passaging. We do not recommend customer to re-freeze the astrocytes.

Technical Resources

References

  • Shaltouki A, Peng J, Liu Q, Rao M & Zeng X (2013) Efficient Generation of Astrocytes from Human Pluripotent Stem Cells in Defined Conditions