Human iPSC-Derived Mature Astrocyte Kit

1680.00

Kit includes: 1 million iPSC-Derived Mature Astrocytes and 1 x 100 mL bottle of Astrocyte Maintenance Medium (including Supplement A, Supplement B and Supplement C)

Product Code: ax0084 (1 kit) Categories: , .

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Description

Axol iPSC-Derived Mature Astrocyte Kit contains cryopreserved mature astrocytes together with optimized Astrocyte Maintenance Medium to maintain the astrocytes. The astrocytes are derived from an iPSC line from a healthy donor. More than 80% of the cells are GFAP+ (Glial Fibrillary Acidic Protein) and ≤15% of the cells are TUJ1+ (neuronal class III ß-tubulin) neurons. 

Comparing marker expression in iPSC-derived and primary astrocytes using immunocytochemistry

Expression of GFAP, S100, nestin, MAP2, SOX2, ALDH1L1, glutamine synthetase, EAAT1 and integrin beta 5 in our iPSC-derived mature astrocytes, competitor cells and primary astrocytes. (Data provided by Susanne Zach, Boehringer Ingelheim.)

click here to see the full data set

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A2 A3 B35(Bw6) B44(Bw4) Cw4 Cw5
Karyotype Normal
Reprogramming method Episomal vector
Induction method Fully defined medium
Genetic modification None
Size 1 million cells
Kit components 1 vial of iPSC-Derived Mature Astrocytes (≥1 million cells), 1 x 50 mL bottle of Astrocyte Maintenance Medium (including Supplement A, Supplement B and Supplement C)
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen

Technical Resources

References

  • Read nowShaltouki A, Peng J, Liu Q, Rao M & Zeng X (2013) Efficient Generation of Astrocytes from Human Pluripotent Stem Cells in Defined Conditions