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Human iPSC-derived Mature Microglia, Cryopreserved

ax0668: 1 x vial of Human iPSC-derived Mature Microglia; Cryopreserved > 1 million cells per vial

Post thaw viability: 85 - 90%


Axol recommends both Surebond-XF and Concanavalin in order to attach to cell culture surfaces. They are not amenable to other coating substrates. 

Product Code: ax0668 Categories: , .

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Description

Phenotypic Characterization

All markers are well expressed at protein level.

Performed 3 days post thaw of frozen vial. 

Functional Phagocytosis data

Phagocytosis is used as a primary measure of functional Microglia activity.  The Cryopreserved Microglia (ax0668) exhibit substrate-dependent phagocytosis when exposed to fluorescent pHrodo labelled beta-amyloid. The Human IPSC derived cryopreserved microglia are shown to be highly phagocytic in nature and equivalent to fresh cells.

Courtesy of Medicine Discovery Catapult; Alderley Edge, Cheshire, UK

Product Specification

Culture properties Adherent
Bio-Safety Level Axol recommends: Level 2
Starting material Cord blood CD34+ cells
Donor gender Female
Donor age at sampling Newborn
Reprogramming method Episomal
Induction method Fully defined medium
Genetic modification None
Size ≥1 million cells per vial
Cell type hiPSC-derived Cryopreserved Mature Microglia
Shipping conditions Dry ice
Storage conditions Vapour phase nitrogen

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