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Human iPSC-Derived Motor Neuron Progenitors

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Description

Axol Human iPSC-Derived Motor Neuron Progenitors are derived from a healthy male donor. 

Axol iPSCs were differentiated to motor neuron progenitors using a combination of small molecules to regulate multiple signalling pathways. Initial expansion of iPSC-Motor Neuron Progenitors is possible before the terminal differentiation to motor neurons. 

Axol Human iPSC-Derived Motor Neurons have been characterised by ICC and the motor neurons after 14 days in culture express HB9, LIM3, OLIG2 and TUJ1. After a further 14 days they express choline acetyltransferase (ChAT) and islet-1 (ISL1).

Product Specification

Starting material Fibroblasts
Donor gender Male
Donor age at sampling 74 yrs
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size ≥2 million cells
Kit components 1 vial of Motor Neuron Progenitors (≥2 million cells)
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

Frequently Asked Questions

You could expand the motor neuron progenitors in a T25 flask. Passage when culture reach 70% confluence, and re-plate at a density no greater than 150,000 cells/cm2. Ideal seeding density is 100,000 cells/cm2 after passaging.

Always filter the media after retinoic acid (RA) addition. Do not subject the RA aliquots to multiple freeze thaws RA is extremely light sensitive so protect the media with RA by wrapping the bottle/tube with foil.

For best results, use Surebond + Readyset for final plating.

Technical Resources