Axol Human iPSC-Derived Motor Neuron Progenitors are derived from a healthy male donor.
Axol iPSCs were differentiated to motor neuron progenitors using a combination of small molecules to regulate multiple signalling pathways. Initial expansion of iPSC-Motor Neuron Progenitors is possible before the terminal differentiation to motor neurons.
Axol Human iPSC-Derived Motor Neurons have been characterised by ICC and the motor neurons after 14 days in culture express HB9, LIM3, OLIG2 and TUJ1. After a further 14 days they express choline acetyltransferase (ChAT) and islet-1 (ISL1).
|Donor age at sampling
||Monolayer & chemically defined medium
||≥2 million cells
||1 vial of Motor Neuron Progenitors (≥2 million cells)
||vapour phase nitrogen
Frequently Asked Questions
Can I expand and passage motor neuron progenitors?
You could expand the motor neuron progenitors in a T25 flask. Passage when culture reach 70% confluence, and re-plate at a density no greater than 150,000 cells/cm2. Ideal seeding density is 100,000 cells/cm2 after passaging.
How do I handle medium with Retinoic Acid supplement?
Always filter the media after retinoic acid (RA) addition.
Do not subject the RA aliquots to multiple freeze thaws
RA is extremely light sensitive so protect the media with RA by wrapping the bottle/tube with foil.
What coating reagent should I use for Motor Neuron cultures?
For best results, use Surebond + Readyset for final plating.