Axol's Human iPSC-Derived Motor Neuron Progenitors from a healthy male donor carrying C9orf72 hexanucleotide expansion. The repeat expansion of the parental iPSC line of the motor neuron progenitor was determined by Southern blot.
C9orf72 is present in ~ 40% of familial ALS and 8-10 % of sporadic ALS. It is the most common mutation related to ALS - far more common than SOD1 or TDP-43.
||C9ORF72 repeat expansion
|Donor age at sampling
||≥2 million cells
Frequently Asked Questions
Can I expand and passage motor neuron progenitors?
You could expand the motor neuron progenitors in a T25 flask. Passage when culture reach 70% confluence, and re-plate at a density no greater than 150,000 cells/cm2. Ideal seeding density is 100,000 cells/cm2 after passaging.
How do I handle medium with Retinoic Acid supplement?
Always filter the media after retinoic acid (RA) addition.
Do not subject the RA aliquots to multiple freeze thaws
RA is extremely light sensitive so protect the media with RA by wrapping the bottle/tube with foil.
What coating reagent should I use for Motor Neuron cultures?
For best results, use Surebond + Readyset for final plating.