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Human iPSC-Derived Neural Stem Cells - Huntington's Disease Patient (CAG50)

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Description

Model Huntington's Disease in a Dish

We have applied our innovative technology to develop human neural stem cells directly differentiated from Huntington’s Disease iPS cells.  Our cells allow Huntington's Disease researchers to build their own human cellular models in a disease-relevant genetic and tissue context. 

Our ready-to-use kits allow you to concentrate on your experiments rather than spending valuable time and resources generating Huntington's Disease neural progenitors in-house.  As part of the HD NSC kit, you will receive Plating-XF Medium in addition to your vial of human neural stem cells to maximize cell viability.  

HD NSCs are ready made and easy to differentiate.  They are capable of differentiating into a spectrum of cortical excitatory and inhibitory neurons and remain viable in culture for months, facilitating reproducible and long-term studies.

Whether you want to study Huntington's Disease pathogenesis at the cellular level, perform neurotoxicity testing or pre-clinical drug screening, we believe that HD NSCs, alongside our optimized culture reagents, provide the perfect culture system for your research success.

Odoo image and text block

Phase Contrast Image of Huntington's Disease Human Neural Stem Cells. 

The image was taken at Day 7 in vitro. In the centre of the image you can see HD NSCs in a neural rosette formation. Neurite outgrowth can also be observed. 

Huntington's Disease Neural Stem Cells Express Neural Stem/Progenitor Cell Markers.

HD NSCs were fixed after 7 days in culture and stained using our immunocytochemisty protocol. The neural stem cells were counterstained with DAPI to visualise the nuclei. The cells can be seen to express neural stem/progenitor cell markers Ki67, Nestin and Pax6.

Odoo image and text block                                              DAPI  Pax6  Nestin
Odoo image and text block

                          DAPI Nestin Ki67

Advantages of Axol HD NSCs

  • Human cells derived from diagnosed Huntington's Disease patients

  • Available in industrial quantity

  • High purity neural stem cell population

  • Ready-made and easy to differentiate

  • Robust enough to undergo genetic manipulation

  • Capable of differentiating to a spectrum of cortical excitatory and inhibitory neurons

  • Remain viable in culture for months to facilitate reproducible and long term studies

Applications

 

  • Studying Huntington's Disease pathogenesis at the cellular level

  • Studying neural network formation and signal transmission in Huntington's Disease neural culture

  • Neurotoxicity testing

  • Preclinical drug efficacy testing

  • Co-culture studies with astrocytes


Product Specification

Starting material Fibroblasts
Donor gender Female
Reprogramming method Retroviral
Induction method Monolayer & chemically defined medium
Size ≥1.5 million cells
Kit components 1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Cell type iPSC-derived neural stem cells
Growth properties Adherent
Disease information CAG repeat expansion 50
Shipping conditions Dry ice
Storage conditions Liquid nitrogen

Frequently Asked Questions

For Axols iPSC derived NSCs, Only move into maintenance media when at least 80% of your culture has differentiated into cortical neurons.

Always plate onto Surebond (ax0041) if you intend to detach the cells for continued expansion

Plate the cells at a low density (recommend 70k/cm2) on Readyset + Surebond (ax0052)

Surebond-XF - Xeno-free coating reagent needed for endpoint assays on plastic, this is the easiest method of coating, with no wash steps. Surebond + Readyset - Coating reagent needed for endpoint assays on glass Surebond- Coating reagent needed when passaging of NSCs is required

References

  • Wevers NR, van Vught R, Wilschut KJ, et al., High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform. Scientific Reports (2016)