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Description
Model Huntington's Disease in a Dish
We have applied our innovative technology to develop human neural stem cells directly differentiated from Huntington’s Disease iPS cells. Our cells allow Huntington's Disease researchers to build their own human cellular models in a disease-relevant genetic and tissue context.
Our ready-to-use kits allow you to concentrate on your experiments rather than spending valuable time and resources generating Huntington's Disease neural progenitors in-house. As part of the HD NSC kit, you will receive Plating-XF Medium in addition to your vial of human neural stem cells to maximize cell viability.
HD NSCs are ready made and easy to differentiate. They are capable of differentiating into a spectrum of cortical excitatory and inhibitory neurons and remain viable in culture for months, facilitating reproducible and long-term studies.
Whether you want to study Huntington's Disease pathogenesis at the cellular level, perform neurotoxicity testing or pre-clinical drug screening, we believe that HD NSCs, alongside our optimized culture reagents, provide the perfect culture system for your research success.
Phase Contrast Image of Huntington's Disease Human Neural Stem Cells.
The image was taken at Day 7 in vitro. In the centre of the image you can see HD NSCs in a neural rosette formation. Neurite outgrowth can also be observed.
Huntington's Disease Neural Stem Cells Express Neural Stem/Progenitor Cell Markers.
HD NSCs were fixed after 7 days in culture and stained using our immunocytochemisty protocol. The neural stem cells were counterstained with DAPI to visualise the nuclei. The cells can be seen to express neural stem/progenitor cell markers Ki67, Nestin and Pax6.
DAPI Pax6 Nestin
DAPI Nestin Ki67
Advantages of Axol HD NSCs
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Human cells derived from diagnosed Huntington's Disease patients
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Available in industrial quantity
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High purity neural stem cell population
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Ready-made and easy to differentiate
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Robust enough to undergo genetic manipulation
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Capable of differentiating to a spectrum of cortical excitatory and inhibitory neurons
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Remain viable in culture for months to facilitate reproducible and long term studies
Applications
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Studying Huntington's Disease pathogenesis at the cellular level
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Studying neural network formation and signal transmission in Huntington's Disease neural culture
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Neurotoxicity testing
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Preclinical drug efficacy testing
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Co-culture studies with astrocytes
Product Specification
| Starting material | Fibroblasts |
| Donor gender | Female |
| Reprogramming method | Retroviral |
| Induction method | Monolayer & chemically defined medium |
| Size | ≥1.5 million cells |
| Kit components | 1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL) |
| Cell type | iPSC-derived neural stem cells |
| Growth properties | Adherent |
| Disease information | CAG repeat expansion 50 |
| Shipping conditions | Dry ice |
| Storage conditions | Liquid nitrogen |
Frequently Asked Questions
When do I switch from differentiation media to Maintenance media?
For Axols iPSC derived NSCs, Only move into maintenance media when at least 80% of your culture has differentiated into cortical neurons.
I want to passage and expand the iPSC derrived Neural stem cells, what coating shall I use?
Always plate onto Surebond (ax0041) if you intend to detach the cells for continued expansion
I want to carry out imaging/confocal microscopy, how should I plate the cells?
Plate the cells at a low density (recommend 70k/cm2) on Readyset + Surebond (ax0052)
I am not sure what coating I should use for iPSC derrive Neural stem cells?
Surebond-XF - Xeno-free coating reagent needed for endpoint assays on plastic, this is the easiest method of coating, with no wash steps. Surebond + Readyset - Coating reagent needed for endpoint assays on glass Surebond- Coating reagent needed when passaging of NSCs is required