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Human iPSC-Derived Neural Stem Cells (Male)

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Description

Axol Human iPSC-Derived Neural Stem Cells

Axol Human iPSC-Derived Neural Stem Cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions. The NSCs express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6, FOXG1 and nestin, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture (see below). Additionally, Axol NSCs are capable of generating a spectrum of cerebral cortical excitatory and inhibitory neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of 40 ~ 50 days.

Axol NSCs are easy to differentiate to neurons or mixed neural cell types, following our protocols and using our tailored media and reagent bundles. A highly pure population of neurons can be generated from Axol NSCs following the synchronous differentiation protocol. Using our specialized coating reagents, neurons derived from Axol NSCs can be maintained in culture long-term (>1 year). NSCs are available from multiple donors to suit your research needs and have been characterized extensively.

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A2 A3 B35(Bw6) B44(Bw4) Cw4 Cw5
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size ≥1.5 million cells
Kit components 1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen

Frequently Asked Questions

For Axols iPSC derived NSCs, Only move into maintenance media when at least 80% of your culture has differentiated into cortical neurons.

Always plate onto Surebond (ax0041) if you intend to detach the cells for continued expansion

Plate the cells at a low density (recommend 70k/cm2) on Readyset + Surebond (ax0052)

Please find attached document.

Surebond-XF - Xeno-free coating reagent needed for endpoint assays on plastic, this is the easiest method of coating, with no wash steps. Surebond + Readyset - Coating reagent needed for endpoint assays on glass Surebond- Coating reagent needed when passaging of NSCs is required

Each line will need to be assessed to understand it's growth Profile, lines vary however you should aim to passage no more than 2 times Always allow the cells to recover from mitogen treatment for at least a couple of days before going into differentiation. You do not want to differentiate when cells appear flat. Let the cells recover from the flat morphology and assume NSC like morphology before changing media to diff-XF.

Technical Resources

References

  • A. Odawara, H. Katoh, N. Matsuda, I. Suzuki. Induction of long-term potentiation and depression phenomena in human induced pluripotent stem cell-derived cortical neurons, Biochemical and Biophysical Research Communications (2015).