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Description
Axol Human iPSC-Derived Neural Stem Cells
Axol Human iPSC-Derived Neural Stem Cells (NSCs) are derived from integration-free, induced pluripotent stem cells (iPSCs) under fully defined neural induction conditions. The NSCs express typical markers of cerebral cortical neural stem and progenitor cells such as PAX6, FOXG1 and nestin, and spontaneously form polarized neural tube-like rosette structures when plated as a monolayer in culture (see below). Additionally, Axol NSCs are capable of generating a spectrum of cerebral cortical excitatory and inhibitory neurons that are electrically active and have the ability to form functional synapses and circuits in vitro. After thawing and plating, the neural stem cells terminally differentiate to acquire mature electrophysiological properties, and form functional synaptic networks over a period of 40 ~ 50 days.
Axol NSCs are easy to differentiate to neurons or mixed neural cell types, following our protocols and using our tailored media and reagent bundles. A highly pure population of neurons can be generated from Axol NSCs following the synchronous differentiation protocol. Using our specialized coating reagents, neurons derived from Axol NSCs can be maintained in culture long-term (>1 year). NSCs are available from multiple donors to suit your research needs and have been characterized extensively.
Product Specification
| Starting material | Fibroblasts |
| Donor gender | Male |
| Donor age at sampling | 74 yrs |
| Karyotype | Normal |
| Reprogramming method | Episomal vector |
| Induction method | Monolayer & chemically defined medium |
| Genetic modification | None |
| Size | ≥1.5 million cells |
| Kit components | 1 vial of Neural Stem Cells (≥1.5 million cells) and 1 bottle of Neural Plating-XF Medium (30 mL) |
| Growth properties | Adherent |
| Shipping conditions | Dry ice |
| Storage conditions | Liquid nitrogen |
Frequently Asked Questions
When do I switch from differentiation media to Maintenance media?
For Axols iPSC derived NSCs, Only move into maintenance media when at least 80% of your culture has differentiated into cortical neurons.
I want to passage and expand the iPSC derrived Neural stem cells, what coating shall I use?
Always plate onto Surebond (ax0041) if you intend to detach the cells for continued expansion
I want to carry out imaging/confocal microscopy, how should I plate the cells?
Plate the cells at a low density (recommend 70k/cm2) on Readyset + Surebond (ax0052)
What are the APOE genotypes of the Alzheimers disease Neural Stem Cell products?
Please find attached document.
I am not sure what coating I should use for iPSC derrive Neural stem cells?
Surebond-XF - Xeno-free coating reagent needed for endpoint assays on plastic, this is the easiest method of coating, with no wash steps. Surebond + Readyset - Coating reagent needed for endpoint assays on glass Surebond- Coating reagent needed when passaging of NSCs is required
How long do I need to expand the cells before I start differentiating the cells?
Each line will need to be assessed to understand it's growth Profile, lines vary however you should aim to passage no more than 2 times Always allow the cells to recover from mitogen treatment for at least a couple of days before going into differentiation. You do not want to differentiate when cells appear flat. Let the cells recover from the flat morphology and assume NSC like morphology before changing media to diff-XF.
Technical Resources
References
- Poulsen ET, Iannuzzi F, Rasmussen HF, et al., An aberrant phosphorylation of amyloid precursor protein tyrosine regulates its trafficking and the binding to the clathrin endocytic complex in neural stem cells of Alzheimer's disease patients. Journal of Frontiers in Molecular Neuroscience (2017)
- Zollo A, Allen Z, Rasmussen H, et al., Sortilin-Related Receptor Expression in Human Neural Stem Cells Derived from Alzheimer’s Disease Patients Carrying the APOE Epsilon 4 Allele. Neural Plasticity (2017)