The All-in-One Starter Kit for Human iPSC-derived Sensory Neurons
(Please be aware that the correct volume of Neural Plating Medium ax0033 (not Xeno-Free) needed for your specific order volume of cells will be supplied FREE OF CHARGE)
The addition of the new MATURATION MAXIMIZER supplement to the sensory neuron kit gives:
SIGNIFICANTLY INCREASED KEY MARKER EXPRESSION & ACCELERATE NEURONAL MATURATION
An average increase of 30-45% of neurons expressing TRPv1, Nav1.7 and Nav1.8 in week 4 of culture; 90% neurons response to capsaicin on day 22.
FASTER MATURATION, QUICKER RESULTS
ax0058 MATURATION MAXIMIZER Supplement can only be used with Axol's Human iPSC-derived Sensory Neuron Progenitors (ax0055 or ax0555)
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Description
We have put together our hiPSC-Sensory Neuron Progenitors with our new optimized media including the MATURATION MAXIMIZER supplement with the required growth factors in one kit with an easy-to-follow protocol to help you generate the best sensory neuron culture for your research.
This all in one starter kit contains:
- ax0055 - Human iPSC-Derived Sensory Neuron Progenitors
- ax0060 - Sensory Neuron Maintenance Medium
- ax0058 - Sensory MATURATION MAXIMIZER supplement
- ax139855 - Recombinant Human Glial-Derived Neurotrophic Factor (GDNF)
- ax139800 - Recombinant Human Brain-Derived Neurotrophic Factor (BDNF)
- ax139789 - Recombinant Human Nerve Growth Factor (NGF)
- ax139811 - Recombinant Human Neurotrophin-3 (NT-3)
- ax0053 - SureBond-XF Coating Substrate
MATURATION MAXIMIZER is a fully defined medium supplement for enhancing the existing Sensory Neuron Maintenance Medium (ax0060). It works by mimicking in vivo signals between sensory neurons and their supporting cells. The supplement contains signaling factors present in the peripheral nervous system and in particular the native environment of sensory neurons. Utilizing this supplement accelerates the maturation of iPSC-derived sensory neurons in vitro.
iPSC-derived sensory neurons treated with the Maximizer express key markers, such as TRPv1, TRPa1, TRPv2, TRPv3 and TRPm8. Kinetic states of e.g. TRPv1 are in a fast response inward rectifying state, to agonists such as Capsaicin. Neuronal responses to capsaicin occur at 1μM for 90% of the sensory neuron population.
Functional Characterization using MEA analysis
The MATURATION MAXIMIZER supplement significantly reduces the time to maturation and gives a more physiological relevant model with neuronal response to capsaicin and menthol after only 22 and 27 days, respectively.
Product Specification
Kit components | Human iPSC-Derived Sensory Neuron Progenitors (ax0055), Sensory Neuron Maintenance Medium (ax0060), Sensory MATURATION MAXIMIZER supplement (ax0058), SureBond-XF (ax0053), Recombinant Human Glial-Derived Neurotrophic Factor (GDNF) (ax139855), Recombinant Human Brain-Derived Neurotrophic Factor (BDNF) (ax139800), Recombinant Human Nerve Growth Factor (NGF) (ax139789) and Recombinant Human Neurotrophin-3 (NT-3) (ax139811) |
Technical Resources
- User Guide - Human iPSC-derived Sensory Neuron Progenitor
- Poster - Effect of MATURATION MAXIMIZER supplement on accelerated maturation of Human iPSC-derived Peripheral Sensory Neurons
- Sensory MATURATION MAXIMIZER Functional Characterization: Capsaicin, Menthol, Temp effects using Calcium imaging & MEA