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Human iPSC-Derived Sensory Neuron Progenitors (Male)

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Axol Human iPSC-Derived Sensory Neuron Progenitors derived from a healthy male donor. Axol's induced pluripotent stem cells (iPSCs) were differentiated to sensory neuron progenitors using small molecule inhibitors. For endpoint assays on plastic, SureBond-XF should be used for coating cell culture surfaces. For endpoint assays on glass, SureBond+ReadySet coating reagent should be used. After thawing and plating, the progenitors need to be growth arrested and then should be matured for approximately 6 weeks prior to endpoint assays. The expression of Nav1.7 should occur at around 4-5 weeks of maturation and Nav1.8 is expressed after approximately 6-8 weeks of maturation in Sensory Neuron Maintenance Medium. 

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A29 A68, B38(Bw4) B44(Bw4), Cw8 Cw12
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size 500,000 cells
Kit components 1 vial of progenitors (>500,000 cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Cell type iPSC-derived sensory neuron progenitors
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

Frequently Asked Questions

70-80% sensory neurons express TRPV1 in our immunostaining.

No, This product is a late stage progenitor and are not proliferative. Also, they need to be treated with an anti-mitotic at day 3 so this will halt any remaining proliferative potential

Sensory neurons can be kept in continuous culture for over 10 months. * Special culture method is not required. The Normal culture protocol can be adhered to.

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