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Description
Axol Human iPSC-Derived Sensory Neuron Progenitors derived from a healthy male donor. Axol's induced pluripotent stem cells (iPSCs) were differentiated to sensory neuron progenitors using small molecule inhibitors. For endpoint assays on plastic, SureBond-XF should be used for coating cell culture surfaces. For endpoint assays on glass, SureBond+ReadySet coating reagent should be used. After thawing and plating, the progenitors need to be growth arrested and then should be matured for approximately 6 weeks prior to endpoint assays. The expression of Nav1.7 should occur at around 4-5 weeks of maturation and Nav1.8 is expressed after approximately 6-8 weeks of maturation in Sensory Neuron Maintenance Medium.
Product Specification
Starting material
Cord blood CD34+ cells
Donor gender
Male
Donor age at sampling
Newborn
HLA serotype
A29 A68, B38(Bw4) B44(Bw4), Cw8 Cw12
Karyotype
Normal
Reprogramming method
Episomal vector
Induction method
Monolayer & chemically defined medium
Genetic modification
None
Size
500,000 cells
Kit components
1 vial of progenitors (>500,000 cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Cell type
iPSC-derived sensory neuron progenitors
Growth properties
Adherent
Shipping conditions
Dry ice
Storage conditions
vapour phase nitrogen
Frequently Asked Questions
What percentage of Sesory neurons express TRPV1?
70-80% sensory neurons express TRPV1 in our immunostaining.
Can I expand iPSC-derived sensory neuron progenitors?
No, This product is a late stage progenitor and are not proliferative. Also, they need to be treated with an anti-mitotic at day 3 so this will halt any remaining proliferative potential
How long can iPSC-derived sensory neuron progenitors be kept in culture?
Sensory neurons can be kept in continuous culture for over 10 months.
* Special culture method is not required. The Normal culture protocol can be adhered to.
Axol Human iPSC-Derived Sensory Neuron Progenitors derived from a healthy male donor. Axol's induced pluripotent stem cells (iPSCs) were differentiated to sensory neuron progenitors using small molecule inhibitors. For endpoint assays on plastic, SureBond-XF should be used for coating cell culture surfaces. For endpoint assays on glass, SureBond+ReadySet coating reagent should be used. After thawing and plating, the progenitors need to be growth arrested and then should be matured for approximately 6 weeks prior to endpoint assays. The expression of Nav1.7 should occur at around 4-5 weeks of maturation and Nav1.8 is expressed after approximately 6-8 weeks of maturation in Sensory Neuron Maintenance Medium.
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