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Human iPSC-Derived Sensory Neuron Progenitors (Male)

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Description

Axol Human iPSC-Derived Sensory Neuron Progenitors derived from a healthy male donor. Axol's induced pluripotent stem cells (iPSCs) were differentiated to sensory neuron progenitors using small molecule inhibitors. For endpoint assays on plastic, SureBond-XF should be used for coating cell culture surfaces. For endpoint assays on glass, SureBond+ReadySet coating reagent should be used. After thawing and plating, the progenitors need to be growth arrested and then should be matured for approximately 6 weeks prior to endpoint assays. The expression of Nav1.7 should occur at around 4-5 weeks of maturation and Nav1.8 is expressed after approximately 6-8 weeks of maturation in Sensory Neuron Maintenance Medium. 

Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
HLA serotype A29 A68, B38(Bw4) B44(Bw4), Cw8 Cw12
Karyotype Normal
Reprogramming method Episomal vector
Induction method Monolayer & chemically defined medium
Genetic modification None
Size 500,000 cells
Kit components 1 vial of progenitors (>500,000 cells) and 1 bottle of Neural Plating-XF Medium (30 mL)
Cell type iPSC-derived sensory neuron progenitors
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

Frequently Asked Questions

Yes, it is normal. The mitomycin treatment step will eliminate the flat cells.

Expect significant cell death post mitomycin C treatment. Mitomycin's effect becomes very evident a couple of days after treatment and this is fine. Don't panic if you see residual mitomycin c induced cell death even 8-10 days post-treatment. If your sensory neuron culture is maintained in a healthy way throughout the differentiation process you will have high-quality networked sensory neurons 2 weeks after thawing.

Yes, this is a necessary purification step. If you do not treat the cells with mitomycin C, then the flat non-sensory neuron cells would take over the culture.

Please make sure you change medium gently and avoid adding the medium from one side of the wells throughout the 5-6 weeks of culture. If the cells start to peel from the corners, it can be repaired by adding Surebond (ax0041) into your standard feeding media. Usually, we use 120 uL Surebond in 12 mL medium for a few days until the layer re-attach. This can be applied no matter what coating has been used.

They work best on Readyset + Surebond (our strongest coating set). They are OK on Surebond-XF but mature cells tend to tug at each other pulling the layer apart, which surebond-XF can not stop or prevent.

For terminally differentiated cultures of sensory neurons, you can change medium every 2 to 3 days.

Yes.

No, these cells would need to be treated with mitomycin C at day 3 which would halt any remaining proliferative potential.

After 5 weeks of differentiation, 70-80% of terminally differentiated sensory neurons express TRPV1 as validated with ICC.

Sensory neurons can be kept in continuous culture for over 10 months. Special culture method is NOT required. The standard culture protocol and Sensory neuron maintenance medium can be used for supporting the long term culture.

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