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Human iPSC-Derived Ventricular Cardiomyocytes (Male)

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Description

Human iPSC-Derived Ventricular Cardiomyocytes (1 million cells)

The induced pluripotent stem cells (iPSCs) used to generate Human iPSC-Derived Ventricular Cardiomyocytes (male, ax2520) have been well characterized and used widely within the research community. For iPSC characterization information please see ‘Baghbaderani BA, Tian X, Neo BH, et al. cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications. Stem Cell Reports (2015)’. 

CD34+ cord blood cells were reprogrammed using episomal vectors under current good manufacturing practice (cGMP) conditions to produce a master bank of iPSCs. A non-GMP, research grade working bank of iPSCs were created from the master stock of cGMP-grade iPSCs for research studies (Baghbaderani et al., 2015). Axol has differentiated these matching research grade iPSCs into ventricular cardiomyocytes, as such these Human iPSC-Derived Ventricular Cardiomyocytes can be used for pre-clinical applications.



Product Specification

Starting material Cord blood CD34+ cells
Donor gender Male
Donor age at sampling Newborn
Reprogramming method Episomal vector
Genetic modification None
Size 1 million cells
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions vapour phase nitrogen

Frequently Asked Questions

Beat rate may vary depending on the plating density. You want to always wait till the Ventricular cardiomyocyte culture is confluent before assessing the beat rate.

Technical Resources

References

  • Shen Y, Dana H, Abdelfattah A.S et al., A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein Biorxiv (2018)
  • Sutton K, Ridly J, El Haou S et al., Human Stem Cell-Derived Cardiomyocytes: In Vitro Assays and Screening Platforms for Exploring Ventricular and Atrial Phenotypes Journal of Pharmacological and Toxicological Methods (2017)
  • Liquid Crystalline Networks toward Regenerative Medicine and Tissue Repair
  • Papp R, Bett GCL, Lis A et al., Genomic upregulation of cardiac Cav1.2α and NCX1 by estrogen in women. BioMed Central (2017)
  • Zuppinger C, Gibbons G, Dutta-Passecker P et al., Characterization of cytoskeleton features and maturation status of cultured human iPSC-derived cardiomyocytes. European Journal of Histochemistry (2017)
  • Chang Y, Broyles CN, Brook FA et al., Non-invasive phenotyping and drug testing in single cardiomyocytes or beta-cells by calcium imaging and optogenetics. PLoS One (2017)