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Neural Differentiation-XF Medium

Product Code: ax0034-125 Categories: , , .

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Description

Generate a Highly Pure Population of Neurons
in Less Than 5 days

Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.

18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF). 

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36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF). 

54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF). 

Product Specification

Size 125 mL
Shipping conditions Dry ice
Storage conditions -80°C
Xeno free Yes

Frequently Asked Questions

Please switch to the Maintenance Medium-XF after the suggested period of Neural differentiation medium-XF treatment (see page 12 and 15 of the Human iPSC-Derived Neural Stem Cell Master Protocol below).

Please do not passage the NSCs more than 3 times as the cells will transition to gliogenic state over longer term culture. We found that the cells treated with growth factors (EGF and FGF2) gradually adopt a "flat" morphology, however, when the growth factors are removed, they revert back to spindle-like morphology. This is completely normal. It is best to withdraw the growth factors (EGF and FGF2) first (at least a couple of days) before differentiation using neural differentiation medium.

References

  • Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)