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Description
Generate a Highly Pure Population of Neurons in Less Than 5 days
Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.
18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
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36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
Product Specification
Size
125 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Xeno free
Yes
Frequently Asked Questions
For cortical neurogenesis, when do I switch from the Neural Differentiation-XF medium to Neural Maintenance Medium-XF?
Please switch to the Maintenance Medium-XF after the suggested period of Neural differentiation medium-XF treatment (see page 12 and 15 of the Human iPSC-Derived Neural Stem Cell Master Protocol below).
How long can I expand the NSCs before differentiation?
Axol do not recommend the expansion of NSCs prior to differentiation. For best results, thaw the frozen NSC and then begin the differentiation process 24 hours later.
References
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
Generate a Highly Pure Population of Neurons in Less Than 5 days
Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.
18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
.
36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
Size
125 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Xeno free
Yes
Read now
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
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