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Description
Generate a Highly Pure Population of Neurons in Less Than 5 days
Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.
18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
.
36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
Product Specification
Size
125 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Xeno free
Yes
Frequently Asked Questions
For cortical neurogenesis, when do I switch from the Neural Differentiation-XF medium to Neural Maintenance Medium-XF?
Please switch to the Maintenance Medium-XF after the suggested period of Neural differentiation medium-XF treatment (see page 12 and 15 of the Human iPSC-Derived Neural Stem Cell Master Protocol below).
How long can I expand the NSCs before differentiation?
Please do not passage the NSCs more than 3 times as the cells will transition to gliogenic state over longer term culture.
We found that the cells treated with growth factors (EGF and FGF2) gradually adopt a "flat" morphology, however, when the growth factors are removed, they revert back to spindle-like morphology. This is completely normal.
It is best to withdraw the growth factors (EGF and FGF2) first (at least a couple of days) before differentiation using neural differentiation medium.
References
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
Generate a Highly Pure Population of Neurons in Less Than 5 days
Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.
18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
.
36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
Size
125 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Xeno free
Yes
Read now
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
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