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Neural Differentiation-XF Medium

Product Code: ax0034-125 Categories: , , .

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Description

Generate a Highly Pure Population of Neurons
in Less Than 5 days

Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.

18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF). 

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36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF). 

54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF). 

Product Specification

Size 125 mL
Shipping conditions Dry ice
Storage conditions -80°C
Xeno free Yes

Frequently Asked Questions

Nnly move into maintenance media (NMM-XF) when at least 80% of your culture has differentiated to the cortical neurons.

Each line will need to be assessed to understand it's growth Profile, lines vary however you should aim to passage no more than 2 times Always allow the cells to recover from mitogen treatment for at least a couple of days before going into differentiation. You do not want to differentiate when cells appear flat. Let the cells recover from the flat morphology and assume NSC like morphology before changing media to diff-XF.

References

  • Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)