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Description
Generate a Highly Pure Population of Neurons in Less Than 5 days
Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.
18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
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36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
Product Specification
Size
125 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Xeno free
Yes
Frequently Asked Questions
When do I switch from differentiation media to Maintenance media?
For Axols iPSC derived NSCs, Only move into maintenance media when at least 80% of your culture has differentiated into cortical neurons.
How long do I need to expand the cells before I start differentiating the cells?
Each line will need to be assessed to understand it's growth Profile, lines vary however you should aim to passage no more than 2 times
Always allow the cells to recover from mitogen treatment for at least a couple of days before going into differentiation. You do not want to differentiate when cells appear flat. Let the cells recover from the flat morphology and assume NSC like morphology before changing media to diff-XF.
References
Wang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
Generate a Highly Pure Population of Neurons in Less Than 5 days
Used in combination with our Axol Neural Maintenance-XF Medium, we provide a quick and easy protocol through which users are able to consistently select for a highly pure (>95%) population of neurons with mature morphological and electrical features.
18 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
.
36 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
54 hours after treatment of Axol Neural Stem Cells with Axol Neural Differentiation-XF Medium (ax0034-125) on Sure Bond-XF coating (ax0041XF).
Size
125 mL
Shipping conditions
Dry ice
Storage conditions
-80°C
Xeno free
Yes
Read nowWang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
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