Axol's Neural Maintenance-XF Medium is a fully defined, xeno-free cell culture medium specially formulated for optimal maintenance of neural stem cells and neural cell types. The maintenance medium supports axonal elongation, synaptic activity and reduces neuronal clumping making it suitable for long-term culture of neurons.
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Description
Next-generation neural cell culture medium restricted to animal-free components. It has been optimized to support low density culture and long-term functional maturation.
Xeno-Free Cell Culture Medium for optimal maintenance of
human neural stem cells and neural cell types
For long-term neuronal cultures, Axol's Neural Maintenance-XF Medium provides an ideal environment to support axonal elongation and synaptic activity. Neural Stem Cells cultured in this medium maintain the capacity to differentiate into neuronal and glial lineages. The medium is completely defined ensuring lot-to-lot consistency and reliable performance between experiments. Neural Maintenance-XF Medium can maintain healthy neurons when cultured at low density (10,000 cells/cm2).
Neural Stem Cells were cultured on a SureBond+ReadySet coated culture vessel using Neural Differentiation-XF Medium. 54 h after thawing, the NSCs cells were switched to Neural Maintenance-XF Medium. The resulting neurons were stained for TUJ1 expression and counterstained using DAPI. Image taken at Day 14.
Neural Stem Cells were cultured on a SureBond+ReadySet coated culture vessel using Neural Differentiation-XF Medium. 54 h after thawing, the neural stem cells were switched to Neural Maintenance-XF Medium. The resulting neurons were stained using an antibody targeting MAP2. The cells were counterstained using DAPI. Image taken at Day 14.
Neural Stem Cells were briefly expanded in Neural Expansion-XF Medium and passaged onto a SureBond+ReadySet coated culture vessel. The cells were maintained in Neural Maintenance-XF Medium. The cells were counterstained using DAPI. Neural stem cells can be visualized as Ki67-positive cells. Neurons differentiated from the neural stem cells are shown using the marker TUJ1. Image taken at Day 7.
Product Specification
| Size | 500 mL |
| Shipping conditions | Dry ice |
| Storage conditions | -80°C |
| Xeno free | Yes |
References
- Read nowWang W, Wang L, Lu, J, et al., The inhibition of TDP-43 mitochondrial localization blocks its neuronal toxicity. Nature Medicine (2016)
- Read nowWevers NR, van Vught R, Wilschut KJ, et al., High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform. Scientific Reports (2016)
