Parental MCF7 Cells (MCF7/S0.5)

800.00
Parental MCF7 Cells (MCF7/S0.5) (1 million cells/vial)
Product Code: ax4010 Categories: , .

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MCF7/S0.5 was adapted to grow in low-serum culture medium to reduce estrogen exposure from fetal bovine serum. This permits the cells to be used for experiments studying hormone signaling and the effects of drugs that target hormone receptors. This line was used as the parental line from which the tamoxifen-resistant and fulvestrant-resistant MCF7 cell lines were generated. Therefore, drug sensitive and resistant matched cell lines are available.

Condition of sale: the end user is not permitted to transfer or re-freeze the cells. 
Donor gender Female
Donor age at sampling 69 yrs
Genetic modification None
Size 1 million cells
Growth properties Adherent
Disease information Malignant adenocarcinoma derived from pleural effusion
General notes Expresses the estrogen receptor (ER)
Shipping conditions Dry ice
Storage conditions Liquid nitrogen
Usage notes Culture in DMEM/F12 medium (no phenol red) supplemented with 1% fetal bovine serum + 2.5 mM GlutaMAX + 6 ng/mL insulin
Condition of sale The end user is not permitted to transfer or re-freeze the cells
  • Read nowBriand P, Lykkesfeldt AE. Effect of estrogen and antiestrogen on the human breast cancer cell line MCF-7 adapted to growth at low serum concentration. Cancer Research (1984)
  • Read nowLykkesfeldt AE, Briand P. Indirect mechanism of oestradiol stimulation of cell proliferation of human breast cancer cell lines. British Journal of Cancer (1986)
  • Read nowLykkesfeldt AE, Laursen I, Briand P. Regulation of the secretion of proteins synthesized by the human breast cancer cell line, MCF-7. Molecular and Cellular Endocrinology (1989)
  • Read nowLykkesfeldt AE, Madsen MW, Briand P. Altered expression of estrogen-regulated genes in a tamoxifen-resistant and ICI 164,384 and ICI 182,780 sensitive human breast cancer cell line, MCF-7/TAMR-1. Cancer Research (2014)
  • Read nowLykkesfeldt AE, Larsen SS, Briand P. Human breast cancer cell lines resistant to pure anti-estrogens are sensitive to tamoxifen treatment. International Journal of Cancer (1995)
  • Read nowSonne-Hansen K, Lykkesfeldt AE. Endogenous aromatization of testosterone results in growth stimulation of the human MCF-7 breast cancer cell line. J Steroid Biochem Mol Biol. (2005)
  • Read nowThomsen KG, Lyng MB, Elias D et al. Gene expression alterations associated with outcome in aromatase inhibitor-treated ER+ early-stage breast cancer patients. Breast Cancer Research and Treatment (2015)
  • Read nowAlves CL, Elias D, Lyng M et al. High CDK6 Protects Cells from Fulvestrant-Mediated Apoptosis and is a Predictor of Resistance to Fulvestrant in Estrogen Receptor-Positive Metastatic Breast Cancer. Clinical Cancer Research (2016)
  • Read nowJoshi T, Elias D, Stenvang J et al. Integrative analysis of miRNA and gene expression reveals regulatory networks in tamoxifen-resistant breast cancer. Oncotarget (2016)
  • Read nowHole S, Pedersen AM, Hansen SK et al. New cell culture model for aromatase inhibitor-resistant breast cancer shows sensitivity to fulvestrant treatment and cross-resistance between letrozole and exemestane. International Journal of Cancer (2015)