Human iPSC-Derived Macrophages are generated using a fully defined protocol from a factory of human iPSC-derived monocytes. Once the iPSC-derived monocytes are plated and placed into macrophage differentiation medium the monocytes mature into adherent macrophages.
Axol iPSC-Derived Macrophages have been validated in phagocytosis assays. Both LPS (10 ng/mL) and Cytochalasin D (10 M) have been used to increase and decrease phagocytosis activity respectively. The macrophage-specific calcium-binding protein, IBA1 and cell surface markers CD14, CD11b, CD45, typically expressed by macrophages, have been confirmed in Axol iPSC-Derived Macrophages by flow-cytometry and ICC.