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Primary Human Bronchial Epithelial Cells

Primary Human Bronchial Epithelial Cells (PHBEC) are a good source of a mixed cell population for use in an in vitro model for studying the function and pathophysiology of the respiratory epithelium. PHBEC allow researchers to perform FACS sorting of the diverse population of epithelial cells found within the cell composition. These cells also allow for a representative sample of RNA/DNA/Protein from the epithelial cell mix which is representative of individual donors. 



Product Code: ax134465 (500,000 cells) Categories: .

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Features & Benefits

Primary Human Bronchial Epithelial Cells (PHBEC) are isolated from the bronchus at the bifurcation point to the fourth generation of bronchi. The cell composition consists of differentiated ciliated, non-ciliated, goblet, and basal cells. PHBEC have been characterized and Air/Liquid Interface culture validated by immunofluorescence using protein markers for Cilia (acetylated tubulin), mucin (MUC5AC), tight junctions (Zonula Occludens-1), and basal cells (cytokeratin-5). These cells are guaranteed to have minimum population doublings of at least 20 (from P0 to P2) following the instructions and conditions provided by Axol.  

PHBEC are a perfect source for single-cell analysis from individual donors. The cells can be grown with any method amenable to expansion of primary airway epithelia, so if a researcher has a particular need for different culture conditions (e.g. animal-free media), then this is an excellent choice of cell stock.

Different passages are available, please enquire for latest availability. Researchers wanting a representative sample of human airway epithelia should choose P0 cells over P1.

PHBEC are for research use only and are not approved for human or animal use, nor for in vitro diagnostic testing.

Product Specification

Donor age 62
Donor disease N/A
Donor mutation N/A
Cell subtype Primary
Cell source Human Lung
Quantity 500,000 cells/vial
Donor gender Male
HLA serotype N/A
Karyotype Normal
Genetic modification N/A
Growth properties Adherent
Shipping conditions Dry ice
Storage conditions Liquid nitrogen