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Description
Primary
Human Non-Characterised Hepatocytes are isolated from whole human livers
that are not suitable for liver transplantation.
Non-characterized
plateable hepatocytes are evaluated by yield, viability, morphology, and
plating efficiency. Each plateable lot is guaranteed to contain a minimum of 3
million cells per vial, have a viability of > 85%, and maintain > 70%
confluency for a minimum period of 5 days. Non-characterized suspension
hepatocytes are evaluated for post-thaw yield and viability. Each suspension
lot is guaranteed to contain a minimum of 3 million cells per vial and have a
viability of > 75%.
Hepatocyte
lots can also be analysed for purity through FACS analysis for the cell
markers: CD147, CD166, and CD45. All of the lot-specific information will be
reported on the Certificate of Analysis (CoA).
Please
see our protocol/lot dependent for detailed instructions. Primary Human Hepatocytes
protocols do change slightly depending on the lot. Some lots need to have an
additional Percoll step. Culture vessels must be coated with Type I Collagen:
ax3799 is recommend.
Use
ax3754-1 Primary Hepatocyte Thaw Medium and ax3752-1 Primary Hepatocyte
Maintenance Medium for cryosuspension (non plateable) lots. Use
ax3751-1 Primary Hepatocyte Plating Medium for Plateable lots.
All
cells are tested and confirmed negative for HIV-1, HIV-2, HBV and HCV as
detected by PCR. The cells are confirmed to be negative for mycoplasma and
other detectable microbial contamination.
Product Specification
Donor gender
Wide range of donor backgrounds available
Donor age at sampling
Wide range of donor backgrounds available
Size
>3 millions cells/vial *Lot dependent. Please inquire for latest lot quantity and price*
Shipping conditions
Dry ice
Storage conditions
vapour phase nitrogen
Usage notes
Please see our protocol/lot dependent for detailed instructions. Culture vessels must be coated with Type I Collagen: ax3799 is recommend .
Primary
Human Non-Characterised Hepatocytes are isolated from whole human livers
that are not suitable for liver transplantation.
Non-characterized
plateable hepatocytes are evaluated by yield, viability, morphology, and
plating efficiency. Each plateable lot is guaranteed to contain a minimum of 3
million cells per vial, have a viability of > 85%, and maintain > 70%
confluency for a minimum period of 5 days. Non-characterized suspension
hepatocytes are evaluated for post-thaw yield and viability. Each suspension
lot is guaranteed to contain a minimum of 3 million cells per vial and have a
viability of > 75%.
Hepatocyte
lots can also be analysed for purity through FACS analysis for the cell
markers: CD147, CD166, and CD45. All of the lot-specific information will be
reported on the Certificate of Analysis (CoA).
Please
see our protocol/lot dependent for detailed instructions. Primary Human Hepatocytes
protocols do change slightly depending on the lot. Some lots need to have an
additional Percoll step. Culture vessels must be coated with Type I Collagen:
ax3799 is recommend.
Use
ax3754-1 Primary Hepatocyte Thaw Medium and ax3752-1 Primary Hepatocyte
Maintenance Medium for cryosuspension (non plateable) lots. Use
ax3751-1 Primary Hepatocyte Plating Medium for Plateable lots.
All
cells are tested and confirmed negative for HIV-1, HIV-2, HBV and HCV as
detected by PCR. The cells are confirmed to be negative for mycoplasma and
other detectable microbial contamination.
Donor gender
Wide range of donor backgrounds available
Donor age at sampling
Wide range of donor backgrounds available
Size
>3 millions cells/vial *Lot dependent. Please inquire for latest lot quantity and price*
Shipping conditions
Dry ice
Storage conditions
vapour phase nitrogen
Usage notes
Please see our protocol/lot dependent for detailed instructions. Culture vessels must be coated with Type I Collagen: ax3799 is recommend .
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