Human Non-Characterised Hepatocytes are isolated from whole human livers
that are not suitable for liver transplantation.
plateable hepatocytes are evaluated by yield, viability, morphology, and
plating efficiency. Each plateable lot is guaranteed to contain a minimum of 3
million cells per vial, have a viability of > 85%, and maintain > 70%
confluency for a minimum period of 5 days. Non-characterized suspension
hepatocytes are evaluated for post-thaw yield and viability. Each suspension
lot is guaranteed to contain a minimum of 3 million cells per vial and have a
viability of > 75%.
lots can also be analysed for purity through FACS analysis for the cell
markers: CD147, CD166, and CD45. All of the lot-specific information will be
reported on the Certificate of Analysis (CoA).
see our protocol/lot dependent for detailed instructions. Primary Human Hepatocytes
protocols do change slightly depending on the lot. Some lots need to have an
additional Percoll step. Culture vessels must be coated with Type I Collagen:
ax3799 is recommend.
ax3754-1 Primary Hepatocyte Thaw Medium and ax3752-1 Primary Hepatocyte
Maintenance Medium for cryosuspension (non plateable) lots. Use
ax3751-1 Primary Hepatocyte Plating Medium for Plateable lots.
cells are tested and confirmed negative for HIV-1, HIV-2, HBV and HCV as
detected by PCR. The cells are confirmed to be negative for mycoplasma and
other detectable microbial contamination.